Current Genetics

, Volume 18, Issue 6, pp 511–516 | Cite as

Use of the Tn903 neomycin-resistance gene for promoter analysis in the fission yeast Schizosaccharomyces pombe

  • Christine Lang-Hinrichs
  • Claudia Dössereck
  • Isabelle Fath
  • Ulf Stahl
Original Articles

Summary

The bacterial neo gene from transposon Tn903 (Tn601) was used for dominant transformation of the fission yeast Schizosaccharomyces pombe. It was found that high transformation efficiency was dependent on a high level of promoter activity, mediated by the strong promoter of the Schizosaccharomyces pombe alcohol dehydrogenase gene (adh1), as shown by comparing the efficiency of transformation to G418-resistance, the resistance levels of transformed cells, and the in vitro aminoglycoside phosphotransferase activity. On the other hand, the heterologous promoter of the Saccharomyces cerevisiae alcohol dehydrogenase I gene (adc1) is shown to be a weak promoter in Schizosaccharomyces pombe, though its activity is significantly enhanced in cells grown on glycerol as a carbon source. This system for selection and detection of promoter-active sequences may provide a useful basis for the analysis of promoter elements in fission yeast.

Key words

Schizosaccharomyces pombe S. cerevisiae adc1 promoter S. pombe adh1 promoter Aminoglycoside phosphotransferase 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Christine Lang-Hinrichs
    • 1
  • Claudia Dössereck
    • 2
  • Isabelle Fath
    • 1
  • Ulf Stahl
    • 1
    • 2
  1. 1.Institut für Gärungsgewerbe und BiotechnologieBerlin 65Germany
  2. 2.FG Mikrobiologie und GenetikTechnische Universität BerlinBerlin 65Germany

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