Current Genetics

, Volume 27, Issue 1, pp 83–89 | Cite as

Cloning and characterization of a chitinase (CHIT42) cDNA from the mycoparasitic fungus Trichoderma harzianum

  • Irene García
  • José M. Lora
  • Jesús de la Cruz
  • Tahía Benítez
  • Antonio Llobell
  • José A. Pintor-Toro
Original Paper


A cDNA of Trichoderma harzianum (chit42), coding for an endochitinase of 42 kDa, has been cloned using synthetic oligonucleotides corresponding to aminoacid sequences of the purified chitinase. The cDNA codes for a protein of 423 amino acids. Analysis of the N-terminal amino-acid sequence of the chitinase, and comparison with that deduced from the nucleotide sequence, revealed post-translational processing of a putative signal peptide of 22 amino acids and a second peptide of 12 amino acids. The chit42 sequence presents overall similarities with filamentous fungal and bacterial chitinases and to a lesser extent with yeast and plant chitinases. The deduced aminoacid sequence has putative catalytic, phosphorylation and glycosylation domains. Expression of chit42 mRNA is strongly induced by chitin and chitin-containing cell walls and is subjected to catabolite repression. Southern analysis shows that it is present as a single-copy gene in T. harzianum. chit42 is also detected in several tested mycoparasitic and non-mycoparasitic fungal strains.

Key words

Trichoderma Chitinase Mycoparasitism Biological control 


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Irene García
    • 1
  • José M. Lora
    • 1
  • Jesús de la Cruz
    • 2
    • 3
  • Tahía Benítez
    • 4
  • Antonio Llobell
    • 2
    • 3
  • José A. Pintor-Toro
    • 1
  1. 1.Instituto de Recursos Naturales y AgrobiologíaCSICSevillaSpain
  2. 2.Instituto de Bioquímica Vegetal y FotosíntesisCSICSevillaSpain
  3. 3.Universidad de SevillaSevillaSpain
  4. 4.Departamento de GenéticaUniversidad de SevillaSevillaSpain

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