Current Genetics

, Volume 27, Issue 1, pp 38–45

Identification of group-I introns in the 28s rDNA of the entomopathogenic fungus Beauveria brongniartii

  • C. Neuvéglise
  • Y. Brygoo
Original Paper

DOI: 10.1007/BF00326577

Cite this article as:
Neuvéglise, C. & Brygoo, Y. Curr Genet (1994) 27: 38. doi:10.1007/BF00326577

Abstract

The length of the 28s ribosomal DNA differs significantly between two strains (Bt102 and Bt114) of the entomopathogenic fungus Beauveria brongniartii. RFLP analysis on PCR products revealed the presence of three insertional elements of 350–450 bp in strain Bt114. One of the insertions has been cloned and sequenced and shown to possess all the characteristic sequences and secondary structures of a group-IC intron. Its length is 428 bp and it is devoid of any long open reading frame. The distribution of this intron elsewhere in the genome of Bt114, as well as in the chromosomal ribosomal DNA, was studied. It seems to be present as seven copies in different genes not corresponding to the mitochondrial DNA. The presence of the intron in other strains of B. brongniartii was examined by the hybridization method. Some of them seemed to possess introns with a similar core although others presented no homology with the cloned fragment.

Key words

28s rDNA Beauveria brongniartii Group-I intron 

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • C. Neuvéglise
    • 1
  • Y. Brygoo
    • 2
  1. 1.Station de Recherches en Lutte BiologiqueINRAGuyancourFrance
  2. 2.Station de Pathologie VégétaleINRAVersaillesFrance

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