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Molecular and General Genetics MGG

, Volume 206, Issue 1, pp 181–184 | Cite as

Large non-homology in heteroduplex DNA is processed differently than single base pair mismatches

  • C. Dohet
  • S. Džidić
  • R. Wagner
  • M. Radman
Short Communications

Summary

Unmethylated DNA heteroduplexes with a large single stranded loop in one strand have been prepared from separated strands of DNA from two different strains of bacteriophage λ, one of which has a ∼800 base pair IS1 insertion in the cI gene. The results of transfections with these heteroduplexes into wild-type and mismatch repair deficient bacteria indicate that such large non-homologies are not repaired by the Escherichia coli mismatch repair system. However, the results do suggest that some process can act to repair such large non-homologies in heteroduplex DNA. Transfections of a series of recombination and excision repair deficient mutants suggest that known excision or recombination repair systems of E. coli are not responsible for the repair. Repair of large non-homologies may play a role in gene conversion involving large insertion or deletion mutations.

Key words

Escherichia coli Bacteriophage λ DNA loops DNA repair Gene conversion 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • C. Dohet
    • 1
  • S. Džidić
    • 1
  • R. Wagner
    • 1
  • M. Radman
    • 1
  1. 1.Institut Jacques MonodC.N.R.S.-Université Paris VIIParis Cedex 05France

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