Current Genetics

, Volume 22, Issue 6, pp 479–482 | Cite as

Evolutionary analysis of the plastid-encoded gene for the α subunit of the DNA-dependent RNA polymerase of Pyrenomonas salina (cryptophyceae)

  • M. Maerz
  • S. Rensing
  • G. L. Igloi
  • U.-G. Maier
Original Articles
Received:
Accepted:

Abstract

The nucleotide sequence of the gene coding for the plastid-encoded α subunit of DNA-dependent RNA polymerase from the cryptomonad alga Pyrenomonas salina was determined. The deduced amino-acid sequence, corresponding to a 35.2 kDa polypeptide, was compared to homologues from other organisms. Evolutionary relationships were analyzed in detail by the parsimony method together with bootstrap analysis. The deduced phylogenetic tree shows that the cryptomonad gene is the most ancient type of known plastid-encoded RNA polymerase.

Key words

Pyrenomonas salina (Cryptophyceae) Plastid DNA-dependent RNA polymerase α subunit 
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References

  1. Ansorge W, Sproat B, Stegemann J, Schwager C (1986) J Biochem Biophys Methods 13:315–323Google Scholar
  2. Bedwell DM, Davis G, Gosnik M, Post L, Nomura M, Kestler, Zengel JM, Lindahl L (1985) Nucleic Acids Res 13:3891–3903Google Scholar
  3. Berghoefer B, Kroeckel L, Koertner C, Truss M, Schallenberg J, Klein A (1988) Nucleic Acids Res 16:8113–8128Google Scholar
  4. Dunn JJ, Studier FW (1983) J Mol Biol 166:477–535Google Scholar
  5. Felsenstein J (1985) Evolution 39:783–791Google Scholar
  6. Gray MW (1988) Biochem Cell Biol 66:325–348Google Scholar
  7. Greenwood AD (1974) In: Sanders JV, Goodchild DJ (eds) 8th Int Congr Electron Microscopy, Canberra, vol 2, pp 566–567Google Scholar
  8. Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honjii Y, Sun CR, Meng BY, Li YQ, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194Google Scholar
  9. Hird SM, Dyer TA, Gray JC (1989) Nucleic Acids Res 17:6394Google Scholar
  10. Igarashi K, Fujita N, Ishihama A (1990) Nucleic Acids Res 18:5945–5948Google Scholar
  11. Igarashi K, Fujita N, Ishihama A (1991) J Mol Biol 218:1–6Google Scholar
  12. Igloi GL, Kössel H (1992) Crit Rev Plant Sci 10:525–558Google Scholar
  13. Ishihama A (1981) Adv Biophys 14:1–35Google Scholar
  14. Iwabe N, Kuma K, Kishino H, Hasegawa M, Miyata T (1991) J Mol Evol 32:70–78Google Scholar
  15. Leffers H, Gropp F, Lottspeich F, Zillig W, Garett RA (1989) J Mol Biol 206:1–17Google Scholar
  16. Lomardo MJ, Bagga DA, Miller CG (1991) J Bacteriol 173:7511–7518Google Scholar
  17. Ludwig M, Gibbs SP (1985) Protoplasma 127:9–20Google Scholar
  18. Maerz M, Wolters J, Hofmann CJB, Sitte P, Maier U-G (1992) Curr Genet 21:73–81Google Scholar
  19. Maier U-G, Hofmann CJB, Eschbach S, Wolters J, Igloi GL (1991) Mol Gen Genet 230:155–160Google Scholar
  20. Maier RM, Hoch B, Zeltz P, Kössel H (1992) The Plant Cell 4:609–616Google Scholar
  21. McGraw NJ, Bailey JN, Cleaves GR, Dembinski DR, Gocke CR, Joliffe LK, McWright RS, McAllister WT (1985) Nucleic Acids Res 13:6753–6766Google Scholar
  22. Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574Google Scholar
  23. Palenik B, Haselkorn R (1992) Nature 355:265–367Google Scholar
  24. Puehler G, Lottspeich F, Zillig W (1989) Nucleic Acids Res 17:17–4534Google Scholar
  25. Purton S, Gray JC (1989) Mol Gen Genet 217:77–84Google Scholar
  26. Ruf M, Kössel H (1988) Nucleic Acids Res 16:5741–5754Google Scholar
  27. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning; a laboratory manual; Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  28. Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torozawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049Google Scholar
  29. Sibjen-Müller G, Hallik RB, Alt J, Westhoff P, Herrmann RG (1986) Nucleic Acids Res 14:1029–1044Google Scholar
  30. Valentin K, Zetsche K (1990) Plant Mol Biol 15:575–584Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • M. Maerz
    • 1
  • S. Rensing
    • 1
  • G. L. Igloi
    • 2
  • U.-G. Maier
    • 1
  1. 1.Lehrstuhl für ZellbiologieInstitut für Biologie IIFreiburg im BreisgauFederal Republic of Germany
  2. 2.Institut für Biologie IIIFreiburg im BreisgauFederal Republic of Germany

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