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Current Genetics

, Volume 26, Issue 1, pp 79–86 | Cite as

Cloning and characterization of the nuclear gene encoding plastid glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa

  • Yi-Hong Zhou
  • Mark A. Ragan
Original Articles

Abstract

The single-copy nuclear gene (GapA), encoding the plastid-localized glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of the marine red alga Gracilaria verrucosa, has been cloned and sequenced. The GapA transcriptional initiation site was located 49 bp upstream of the start codon, and a putative TATA box was found 54 bp farther upstream. A spliceosomal intron was identified in the transit-peptide-encoding region in a position very similar to intron 1 of GapA and GapB of higher plants; no introns occur in the region encoding the mature protein. These observations provisionally suggest that both red algae and higher plants descend from a single ancestral photosynthetic eukaryote, i.e. that a single endosymbiotic event gave rise to red algal and higher-plant plastids.

Key words

Glyceraldehyde-3-phosphate dehydrogenase gene Gracilaria verrucosa Endosymbiotic origin of plastids Molecular evolution 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Yi-Hong Zhou
    • 1
  • Mark A. Ragan
    • 1
  1. 1.Institute for Marine BiosciencesNational Research Council of CanadaHalifaxCanada

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