Current Genetics

, Volume 26, Issue 1, pp 45–48

Electrophoretic karyotype of Mucor circinelloides

  • Á. Nagy
  • Cs. Vágvölgyi
  • É. Balla
  • L. Ferenczy
Original Articles

DOI: 10.1007/BF00326303

Cite this article as:
Nagy, Á., Vágvölgyi, C., Balla, É. et al. Curr Genet (1994) 26: 45. doi:10.1007/BF00326303

Abstract

Contour-clamped homogeneous electric field (CHEF) gel electrophoresis was used to separate chromosomal size DNA molecules of two Mucor circinelloides strains. Electrophoretic karyotypes revealed the presence of eight distinct bands for the M. circinelloides f. lusitanicus strain, and four, presumably multiple, bands for the M. circinelloides f. gryseo-cyanus strain. The approximate sizes of the resolved chromosomal DNA bands ranged from 2.3 to 8.1 Mb, giving estimated genome sizes of 38.7 and 32.6 Mb, respectively. Hybridisation techniques were used to assign the leuA gene to a chromosome.

Key words

CHEF Electrophoretic karyotype Gene mapping Mucor circinelloides 

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Á. Nagy
    • 1
  • Cs. Vágvölgyi
    • 2
  • É. Balla
    • 1
  • L. Ferenczy
    • 2
  1. 1.Gedeon Richter Chemical WorksBudapest 10Hungary
  2. 2.Department of MicrobiologyAttila József UniversitySzegedHungary

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