Current Genetics

, Volume 20, Issue 3, pp 219–224 | Cite as

Cloning, sequencing and expression of the Schwanniomyces occidentalis NADP-dependent glutamate dehydrogenase gene

  • P. A. De Zoysa
  • I. F. Connerton
  • D. C. Watson
  • J. R. Johnston
Original Articles


The cloned NADP-specific glutamate dehydrogenase (GDH) genes of Aspergillus nidulans (gdhA) and Neurospora crassa (am) have been shown to hybridize under reduced stringency conditions to genomic sequences of the yeast Schwanniomyces occidentalis. Using 5′ and 3′ gene-specific probes, a unique 5.1 kb BclI restriction fragment that encompasses the entire Schwanniomyces sequence has been identified. A recombinant clone bearing the unique BclI fragments has been isolated from a pool of enriched clones in the yeast/E. coli shuttle vector pWH5 by colony hybridization. The idenity of the plasmid clone was confirmed by functional complementation of the Saccharomyces cerevisiae ghd-1 mutation. The nucleotide sequence of the Schw. occidentalis GDH gene, which consists of 1380 nucleotides in a continuous reading frame of 459 amino acids, has been determined. The predicted amino acid sequence shows considerable homology with GDH proteins from other fungi and significant homology with all other available GDH sequences.

Key words

Schwanniomyces occidentalis Expression in Saccharomyces cerevisiae NADP-Glutamate dehydrogenase DNA sequence 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • P. A. De Zoysa
    • 1
    • 2
  • I. F. Connerton
    • 1
  • D. C. Watson
    • 3
  • J. R. Johnston
    • 2
  1. 1.Department of MicrobiologyUniversity of ReadingReadingEngland, UK
  2. 2.Department of Bioscience and BiotechnologyUniversity of StrathclydeGlasgowScotland, UK
  3. 3.Chivas BrothersKeithScotland, UK

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