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Molecular and General Genetics MGG

, Volume 189, Issue 1, pp 48–57 | Cite as

Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12

  • James R. Lupski
  • Bob L. Smiley
  • G. Nigel Godson
Article

Summary

We have fused the E. coli dnaG 5′ regulatory region to the TcR structural gene in the promoter probe plasmids pPV33 and pKK175-6 to demonstrate that a promoter activity is located on a 250 bp SacII-HindIII restriction fragment and that a transcription terminator, previously reported by nucleotide sequence (Smiley et al. 1982) to immediately precede the dnaG gene, acts as such in vivo. We have determined the complete nucleotide sequence of this controlling region and report: 1) tandem promoters on the same SacII-HindIII restriction fragment which promotes tet expression in the gene fusion experiments, 2) an open reading frame between these promoters and the dnaG gene which is the rpsU (ribosomal protein S21) gene, 3) a sequence homologous to the λ nut site, 4) a possible LexA protein binding site on one of the dnaG promoters. This places the order on the E. coli genetic map at 66 min in the clockwise direction as rpsU-dnaG-rpoD which are all contained in a single macromolecular synthesis operon. We postulate a model for regulation of the initiation of DNA replication based on antitermination of the rpsU-dnaG-rpoD operon.

Keywords

Ribosomal Protein Regulatory Region Gene Fusion Complete Nucleotide Sequence Clockwise Direction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Tc

tetracycline

TcR

tetracycline resistance

Ap

ampicillin

bp

base pair

kb

kilobases

term

terminator

P

promoter

nuteq

nut equivalent site

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • James R. Lupski
    • 1
  • Bob L. Smiley
    • 1
  • G. Nigel Godson
    • 1
  1. 1.Department of BiochemistryN.Y.U. Medical CenterNew YorkUSA

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