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Molecular and General Genetics MGG

, Volume 189, Issue 3, pp 471–474 | Cite as

Plasmidic recombination in Escherichia coliK-12: The role of recF gene function

  • Amikam Cohen
  • Avraham Laban
Article

Summary

Interplasmidic and intraplasmidic recombination proficiencies were determined in E. coli bacterial strains carrying rec mutations. Our results defined the role of recF gene function, recB, recC, and sbcB gene products (exonuclease V and exonuclease I) in plasmidic recombination in wild-type E. coli cells and in cells in which the recE recombination pathway is activated. RecF gene function is required for interplasmidic recombination regardless of the recB recC genotype. Intraplasmidic recombination is recF dependent in cells having a functional exonuclease V, but not in recB recC mutants. Exonuclease V activity inhibits both interplasmidic and intraplasmidic recombination via the recE pathway.

Keywords

Recombination Gene Function Gene Product Bacterial Strain Plasmidic Recombination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Amikam Cohen
    • 1
  • Avraham Laban
    • 1
  1. 1.Department of Microbiological ChemistryThe Hebrew University, Hadassah Medical SchoolJerusalemIsrael

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