Molecular and General Genetics MGG

, Volume 189, Issue 3, pp 390–399 | Cite as

Heteroduplex analysis of P-plasmid evolution: The role of insertion and deletion of transposable elements

  • Raimundo Villarroel
  • Robert W. Hedges
  • Ria Maenhaut
  • Jan Leemans
  • Gilbert Engler
  • Marc Van Montagu
  • Jeff Schell


DNA homology of thirteen R-plasmids of group P was examined by heteroduplex analysis and Southern blotting. Ten of these plasmids showed homology for extensive regions including all genes reported as necessary for replication and conjugational transfer. The differences between these plasmids could be explained by gain or loss of DNA sequences, many of which have been shown to be transposons.

Of the other three plasmids, two showed unambiguous homology with the typical P-plasmids but this homology was imperfect, implying that these plasmids are products of lines which have evolved separately for long periods. One plasmid failed to produce heteroduplexes with the reference P plasmid.


Southern Blotting Transposable Element Extensive Region Conjugational Transfer Heteroduplex Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Raimundo Villarroel
    • 1
  • Robert W. Hedges
    • 2
  • Ria Maenhaut
    • 1
  • Jan Leemans
    • 3
  • Gilbert Engler
    • 4
  • Marc Van Montagu
    • 1
    • 3
  • Jeff Schell
    • 1
    • 5
  1. 1.Laboratorium voor GeneticaRijksuniversiteit GentGentBelgium
  2. 2.Plant Genetic SystemsGentBelgium
  3. 3.Laboratorium voor Genetische VirologieVrije Universiteit BrusselSt.-Genesius-RodeBelgium
  4. 4.Laboratorium voor HistologieRijksuniversiteit GentGentBelgium
  5. 5.Max-Planck-Institut für ZüchtungsforschungKöln 30Federal Republic of Germany

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