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Formation of spindle fibers, kinetochore orientation, and behavior of the nuclear envelope during mitosis in endosperm

Fine structural and in vitro studies

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  • 116 Citations

Abstract

The formation of kinetochore (chromosomal) and continuous fibers, and the behavior of the nuclear envelope (NE) was described in studies combining light and electron microscopy. Microtubules (MTs) “push” and “pull” the NE which becomes progressively weaker before breaking. It breaks to a certain extent due to mechanical pressure. Clear zone MTs penetrate into the nuclear area as dense bundles and form continuous fibers. These MTs also attach to some kinetochores during this process. Some kinetochore fibers seem to be formed by the kinetochores themselves which are also responsible for further development and changes of kinetochore fibers. Formation of kinetochore fibers is asynchronous for different chromosomes and even for two sister kinetochores. Often temporary “faulty” connections between different kinetochores or the polar regions are formed which usually break in later stages. This results in movements of chromosomes toward the poles and across the spindle during prometaphase. The NE, whose fine structure has been described, breaks into small pieces which often persist to the next mitosis. Old pieces of NE are utilized in the formation of new NE at telophase. Several problems concerning the mechanism of chromosome movements, visibility of the NE, etc., have also been discussed.

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References

  1. Afzelius, B. A.: The ultrastructure of the nuclear membrane of the sea urchin oocyte as studied with the electron microscope. Exp. Cell Res. 8, 147–158 (1955).

  2. Anderson, E.: The formation of the primary envelope during oocyte differentiation in teleosts. J. Cell Biol. 35, 193–212 (1967).

  3. Bajer, A.: Cine-micrographic studies on mitosis in endosperm. III. Origin of the mitotic spindle. Exp. Cell Res. 13, 493–502 (1957).

  4. Bajer, A.: Cine-miorographic studies on mitosis in endosperm. IV. Exp. Cell Res. 14, 245–256 (1958).

  5. —: Morphological aspects of normal and abnormal mitosis. Konf. Dtsch. Naturf. und Ärzte, Wien, 1965. Probleme der biologischen Reduplikation (ed. P. Sitte), p. 90–119. Berlin-Heidelberg-New York: Springer 1966.

  6. —: Chromosome movement and fine structure of the mitotic spindle. In: Aspects of cell motility. XXIInd Symposium of the Society for Experimental Biology, (ed. H. H. Miller), p. 286–310. London: Cambridge University Press 1968a.

  7. —: Behavior and fine structure of spindle fibers during mitosis in endosperm. Chromosoma (Berl.) 25, 249–281 (1968b).

  8. —: Effects of UV microbeam irradiation on chromosome movements and spindle fine structure. Biophys. J. 9, A-151 (1969).

  9. —, and Molè-Bajer: Cine-micrographic studies on mitosis in endosperm. II. Chromosoma (Berl.) 7, 558–607 (1956).

  10. - -Mitosis and mitotic factors, 16 mm film, 1963.

  11. —: Cine-analysis of some aspects of mitosis in endosperm. In: Cinematography in cell biology (ed. G. G. Rose), p. 357–409. New York: Academic Press 1963.

  12. Beyer, H.: Zur Abbildung extrem dünnwandiger nichtabsorbierender Röhren im Lichtmikroskop. Jena. Jahrb., S. 173–184. Jena: Gustav Fischer 1966.

  13. Branton, D., and H. Moor: Fine structure in freeze-etched Allium cepa L. root tips. J. Ultrastruct. Res. 11, 401–411 (1964).

  14. Brinkley, B. R., and E. Stubblefield: The fine structure of the kinetochore of a mammalian cell in vitro. Chromosoma (Berl.) 19, 28–43 (1966).

  15. Davies, H. G., and J. Tooze: Electron- and light-microscope observations on the spleen of the newt Triturus cristatus: The surface topography of the mitotic chromosomes. J. Cell Sci. 1, 331–350 (1966).

  16. Fisher, H. W., and T. W. Cooper: Electron microscope observations on the nuclear pores of HeLa cells. Exp. Cell Res. 48, 620–622 (1967).

  17. Forer, A.: Characterization of the mitotic traction system, and evidence that birefringent spindle fibers neither produce nor transmit force for chromosome movement. Chromosoma (Berl.) 19, 44–98 (1966).

  18. Franke, W. W.: Isolated nuclear membranes. J. Cell Biol. 31, 619–623 (1966).

  19. —: Zur Feinstruktur isolierter Kernmembranen aus tierischen Zellen. Z. Zellforsch. 80, 585–593 (1967).

  20. Gall, J. G.: An octagonal pattern in the nuclear envelope. J. Cell Biol. 27, 121A, (1965).

  21. —: Octagonal nuclear pores. J. Cell Biol. 32, 391–399 (1967).

  22. Gay, H.: Chromosome-nuclear membrane-cytoplasmic interrelationships in Drosophila. J. biophys. biochem. Cytol. 2, Suppl. 407–414 (1956).

  23. Harris, P.: Some observations concerning metakinesis in sea urchin eggs. J. Cell Biol. 25, 73–77, part 2 (1965).

  24. Inoué, S., and A. Bajer: Birefringence in endosperm mitosis. Chromosoma (Berl.) 5, 48–63 (1961).

  25. Jenkins, R. A.: Fine structure of division in ciliate protozoa. I. micronuclear mitosis in Blepharisma. J. Cell Biol. 34, 463–481 (1967).

  26. Jökelainen, P. T.: The ultrastructure and spatial organization of the metaphase kinetochore in mitotic rat cells. J. Ultrastruct. Res. 19, 19–44 (1967).

  27. Kessel, R. G.: An electron microscope study of nuclear-cytoplasmic exchange in oocytes of Ciona intestinalis. J. Ultrastruct. Res. 15, 181–196 (1966).

  28. —, and H. W. Beams: Nucleolar extrusion in oocytes of Thyone briareus. Exp. Cell Res. 32, 612–615 (1963).

  29. Ledbetter, M. C.: The disposition of microtubules in plant cells during interphas and mitosis. In: Formation and fate of cell organelles. Symposia of the Internat. Soc. for Cell Biology, vol. 6 (Ed. K. B. Warren), p. 55–70. New York: Academic Press 1967.

  30. Luykx, P.: The structure of the kinetochore in meiosis and mitosis in Urechis eggs. Exp. Cell Res. 39, 643–657 (1965a).

  31. —: Kinetochore-to-pole connections during prometaphase of the meiotic divisions in Urechis eggs. Exp. Cell Res. 39, 658–668 (1965b).

  32. Markham, R., S. Frey, and B. J. Hills: Methods for the enhancement of image detail and accentuation of structure in electron microscopy. Virology 20, 88–102 (1963).

  33. Martin, L. C.: The theory of the microscope. New York: Amer. Elsevier Publ. Co. 1966.

  34. Molè-Bajer, J.: Fine structural studies of apolar mitosis. Chromosoma (Berl.) 26, 427–448 (1969).

  35. —, and A. Bajer: Studies of selected endosperm cells with the light and electron microscope. The technique. Cellule 67, 257–265 (1968).

  36. Mollenhauer, H. H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39, 111–114 (1964).

  37. Östergren, G.: The mechanism of co-orientation in bivalents and multivalents. The theory of orientation by pulling. Hereditas (Lund) 37, 85–156 (1951).

  38. —: Mitosis with undivided chromosomes. III. Inhibition of chromosome reproduction in Tradescantia by specific mutations. In: Chromosomes today (ed. C. D. Darlington and K. R. Lewis), p. 128–130. London: Plenum Press 1966.

  39. Porter, K. R., and R. Machado: Mitosis in cells of onion root tips. J. biophys. biochem. Cytol. 7, 167–180 (1960).

  40. Steward, D. L., J. R. Schaeffer, and R. M. Humphry: Breakdown and assembly of polyribosomes in synchronized Chinese hamster cells. Science 161, 791–797 (1968).

  41. Szollosi, O.: Extrusion of nucleoli from pronuclei of the rat. J. Cell Biol. 25, 545–562 (1965).

  42. Vivier, E.: Observations ultrastructurales sur l'envelope nucléaire et ses “pores” chez des sporozoaires. J. de Microsc. 6, 371–390 (1967).

  43. Wada, B.: Analysis of mitosis. Cytologia, vol.30, Suppl. Number (p. 1–158). Tokyo: Internat. Academic Printing Company 1966.

  44. Watson, M. L.: The nuclear envelope. Its. structure and relation to cytoplasmic membranes. J. biophys. biochem. Cytol. 1, 257–270 (1955).

  45. —: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–477 (1958).

  46. Wischnitzer, S.: An electron microscopy study of the nuclear envelope of amphibian oocytes. J. Ultrastruct. Res. 1, 201–222 (1958).

  47. Yasuzumi, G., Y. Nakai, I. Tsubo, M. Yasuda, and T. Sugioka: The fine structure of nuclei as revealed by electron microscopy. IV. The intranuclear inclusion formation in Leydig cells of aging human testes. Exp. Cell Res. 45, 261–276 (1967).

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Bajer, A., Molè-Bajer, J. Formation of spindle fibers, kinetochore orientation, and behavior of the nuclear envelope during mitosis in endosperm. Chromosoma 27, 448–484 (1969). https://doi.org/10.1007/BF00325682

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Keywords

  • Electron Microscopy
  • Developmental Biology
  • Fine Structure
  • Polar Region
  • Nuclear Envelope