Resident macrophages (ED2- and ED3-positive) do not phagocytose degenerating rat skeletal muscle fibres
- 208 Downloads
Two soleus muscles with degenerating muscle fibres were serially sectioned and adjacent sections from various levels of the skeletal muscles were stained with antibodies that react with either monocytes and inflammatory macrophages (ED1) or with the major subpopulations of resident macrophages (ED2 and ED3). ED2+ and ED3+ resident macrophages were abundant throughout the muscles but were not present within the degenerating fibres. ED1+ cells, in contrast, were rarely observed within the undamaged regions of the muscles but were abundant within the degenerating fibres and in the perimysium between arterioles and degenerating fibres. It is concluded that the phagocytosis of damaged muscle fibres is not carried out by the major subpopulations of resident macrophages.
Key wordsSkeletal muscle Macrophages Phagocytosis Degeneration Rat (Wistar)
Unable to display preview. Download preview PDF.
- Adams DO, Hamilton TA (1984) The cell biology of macrophage activation. Annu Rev Immunol 2:283–318Google Scholar
- Barbe E, Damoiseaux JGM, Dopp EA, Dijkstra CD (1990) Characterization and expression of the antigen present on resident macrophages recognized by monoclonal antibody ED2. Immunobiology 182:88–99Google Scholar
- Brown MC, Booth CM, Lunn ER, Perry VH (1991) Delayed response to denervation in muscles of C57BL/O1a mice. Neuroscience 43:279–283Google Scholar
- Damoiseaux JGM, Dopp EA, Beelen RHJ, Dijkstra CD (1989a) Rat bone marrow and monocyte cultures: Influence of culture time and lymphokines on the expression of macrophage differentiation antigens. J Leukoc Biol 46:246–253Google Scholar
- Damoiseaux JGM, Dopp EA, Neefjes JJ, Beelen RHJ, Dijkstra CD (1989b) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kD (CD11/CD18). J Leukoc Biol 46:556–564Google Scholar
- Damoiseaux JGM, Dopp EA, Dijkstra CD (1991) Cellular binding mechanism on rat macrophages for sialylated glycoconjugates, inhibited by the monoclonal antibody ED3. J Leukoc Biol 49:434–441Google Scholar
- Dijkstra CD, Dopp EA, Joling P, Kraal G (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology 54:589–599Google Scholar
- Gordon S, Keshav S, Chung LP (1988) Mononuclear phagocytes: tissue distribution and functional heterogeneity. Curr Opin Immunol 1:26–35Google Scholar
- Grounds MD (1991) Towards understanding skeletal muscle regeneration. Pathol Res Pract 187:1–22Google Scholar
- Honda H, Kimura H, Rostami A (1990) Demonstration and phenotypic characterization of resident macrophages in rat skeletal muscle. Immunology 70:272–277Google Scholar
- Walker R, Bone AJ, Cooke A, Baird JD (1988) Distinct macrophage subpopulations in pancreas of prediabetic BB/E rats: Possible role for macrophages in pathogenesis of IDDM. Diabetes 37:1301–1304Google Scholar