Fresenius' Journal of Analytical Chemistry

, Volume 345, Issue 1, pp 63–67 | Cite as

Simultaneous determination of halogenated benz(a)anthracenes in water by HPLC with fluorometric detection

  • Yasuaki Mori
  • Sumio Goto
  • Sukeo Onodera
  • Syoji Naito
  • Shoji Takitani
  • Hidetsuru Matsushita
Original Papers Environmental Analysis
Received:
Revised:

Summary

A HPLC method using a fluorescence detector is described for the determination of halogenated benz(a)anthracenes (BAX, x=Cl or Br) in water. It consists of the following three procedures; (1) liquid-liquid extraction of BAX with benzene, (2) elimination of interfering compounds from the extracts by one dimensional dual-band thin layer chromatography, and (3) quantitative determination of BAX by HPLC equipped with a fluorescence detector. The recoveries of BAX from water samples through the entire analytical procedure amounted to more than 72%. The calibration curves for BAX were linear, e.g., with a range from 1 to 20 ng ml−1 for 7-chlorobenz(a)anthracene (BACl). The lower detection limit for BACl was 20 pg for an injection volume of 20 μl. This method has been applied to BAX in river water spiked with benz(a)anthracene [B(a)A] at 10 ng ml−1 after chlorination. B(a)A, BACl and BABr were found in the levels of 2.01, 0.16 and 0.13 or less ng ml−1, respectively.

Keywords

HPLC Benzene Detection Limit Water Sample Calibration Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Yasuaki Mori
    • 1
  • Sumio Goto
    • 2
  • Sukeo Onodera
    • 3
  • Syoji Naito
    • 1
  • Shoji Takitani
    • 3
  • Hidetsuru Matsushita
    • 4
  1. 1.Kanagawa Prefectural Public Health LaboratoriesYokohamaJapan
  2. 2.Department of Community Environmental SciencesNational Institute of Public HealthTokyoJapan
  3. 3.Faculty of Pharmaceutical SciencesScience University of TokyoTokyoJapan
  4. 4.Graduate School of Nutritional and Environmental SciencesUniversity of ShizuokaShizuokaJapan

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