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Fresenius' Journal of Analytical Chemistry

, Volume 339, Issue 6, pp 376–383 | Cite as

Multipesticide residue method for fruits and vegetables: California Department of Food and Agriculture

  • S. Mark Lee
  • Michael L. Papathakis
  • Hsiao-Ming C. Feng
  • Gary F. Hunter
  • Joyce E. Carr
Pesticides In Food And Agricultural Products

Summary

This procedure describes the CDFA method for multipesticide residue analysis of fresh fruits and vegetables. An aliquot of chopped sample is blended with acetonitrile. The resulting aqueous acetonitrile extract is filtered and cleaned up via reverse phase solid phase extraction apparatus. The pH of the filtrate is adjusted to neutral using phosphate buffer and the acetonitrile layer is separated from the aqueous layer by a “salting out” process. An aliquot of the acetonitrile layer is concentrated with a K-D evaporator by forming an azeotrope with n-hexane. The prepared sample is assayed for pesticide residues using GLC and HPLC. The performance of this method was evaluated by fortifying 6 representative fruits and vegetables with 7 chlorinated hydrocarbons, 7 organophosphate and 7 N-methylcarbamate pesticides at 0.1–0.2 ppm. No matrix interference was observed and the recovery of residues varied among different samples as well as different pesticides. Chlorothalonil results varied widely with irreproducible recoveries. In general, the method appears to be fast, rugged, and able to detect routinely at the 0.01 ppm level.

Keywords

Solid Phase Extraction Pesticide Residue Fresh Fruit Matrix Interference Chlorothalonil 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • S. Mark Lee
    • 1
  • Michael L. Papathakis
    • 1
  • Hsiao-Ming C. Feng
    • 1
  • Gary F. Hunter
    • 1
  • Joyce E. Carr
    • 1
  1. 1.California Department of Food and Agriculture, Division of Inspection ServicesChemistry Laboratory ServicesSacramentoUSA

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