Fresenius' Journal of Analytical Chemistry

, Volume 340, Issue 4, pp 250–252 | Cite as

Flow-injection determination of HMF in honey by the Winkler method

  • F. Salinas
  • A. Espinosa Mansilla
  • J. J. Berzas Nevado
Food Analysis


A new method for the semiautomatic determination of 5-hydroxymethylfurfural (HMF) by photometric flow injection analysis on the basis of the Winkler's reaction is proposed. The derivative FIA-gram signals were also obtained and detection limit was decreased. The linear range of determination is 2.3–98 μg·ml−1. The presence of sugars is well tolerated. The proposed method can be employed to determine HMF in honey samples with a sampling frequency of 35 h−1.


Sugar Physical Chemistry Analytical Chemistry Inorganic Chemistry Detection Limit 
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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • F. Salinas
    • 1
  • A. Espinosa Mansilla
    • 1
    • 2
  • J. J. Berzas Nevado
    • 2
  1. 1.Department of Analytical ChemistryUniversity of ExtremaduraBadajozSpain
  2. 2.Department of ChemistryUniversity of Castilla-La ManchaCiudad RealSpain

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