, Volume 93, Issue 1, pp 80–87 | Cite as

Genetic differentiation in carbon isotope discrimination and gas exchange in Pseudotsuga menziesii

A common-garden experiment
  • Jianwei Zhang
  • John D. Marshall
  • Barry C. Jaquish
Original Papers


Patterns of genetic variation in gas-exchange physiology were analyzed in a 15-year-old Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) plantation that contains 25 populations grown from seed collected from across the natural distribution of the species. Seed was collected from 33°30′ to 53°12′ north latitude and from 170 m to 2930 m above sea level, and from the coastal and interior (Rocky Mountain) varieties of the species. Carbon isotope discrimination (Δ) ranged from 19.70(‰) to 22.43(‰) and was closely related to geographic location of the seed source. The coastal variety (20.50 (SE=0.21)‰) was not significantly different from the interior variety (20.91 (0.15)‰). Instead, most variation was found within the interior variety; populations from the southern Rockies had the highest discrimination (21.53 (0.20)‰) (lowest water-use efficiency). Carbon isotope discrimination (Δ), stomatal conductance to water vapor (g), the ratio of intercellular to ambient CO2 concentration (ci/ca), and intrinsic water-use efficiency (A/g) were all correlated with altitude of origin (r=0.76, 0.73, 0.74, and −0.63 respectively); all were statistically significant at the 0.01 level. The same variables were correlated with both height and diameter at age 15 (all at P≤0.0005). Observed patterns in the common garden did not conform to our expectation of higher WUE, measured by both A/g and Δ, in trees from the drier habitats of the interior, nor did they agree with published in situ observations of decreasing g and Δ with altitude. The genetic effect opposes the altitudinal one, leading to some degree of homeostasis in physiological characteri tics in situ.

Key words

Pseudotsuga menziesii Genetic differentiation Carbon isotope discrimination Water-use efficiency Altitude 


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  1. Briggs GM, Jurik TW, Gates DM (1986) A comparison of rates of above ground growth and carbon dioxide assimilation by aspen on sites of high and low quality. Tree Physiol 2: 29–34Google Scholar
  2. Condon AG, Richards RA, Farquhar GD (1987) Carbon isotope discrimination is positively correlated with grain yield and dry matter production in field-grown wheat. Crop Sci 27: 996–1001Google Scholar
  3. Craig H (1957) Isotopic standards for carbon and oxygen and correlation factors for mass-spectrometric analysis of carbon dioxide. Geochimica et Cosmochimica Acta 12: 133–149Google Scholar
  4. Ehdaie B, Hall AE, Farquhar GD, Nguyen HT, Waines JG (1991) Water-use efficiency and carbon isotope discrimination in wheat. Crop Sci 31: 1282–1288Google Scholar
  5. Ehleringer JR, White JW, Johnson DA, Brick M (1990) Carbon isotope discrimination, photosynthetic gas exchange, and transpiration efficiency in beans and range grasses. Acta Oecologica 11: 611–625Google Scholar
  6. Farquhar GD, Sharkey TD (1982) Stomatal conductance and photosynthesis. Ann Rev Plant Physiol 33: 317–345Google Scholar
  7. Farquhar GD, O'Leary MH, Berry JA (1982) On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves. Aust J Plant Physiol 9: 121–137Google Scholar
  8. Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40: 503–537Google Scholar
  9. Francey RJ, Gifford RM, Sharkey TD, Weir B (1985) Physiological influences on carbon isotope discrimination in huon pine (Lagarostrobos franklinii). Oecologia 66: 211–218Google Scholar
  10. Friend AD, Woodward FI (1990) Evolutionary and ecophysiological responses of mountain plants to the growing season environment. Adv Ecol Res 20: 59–124Google Scholar
  11. Friend AD, Woodward FI, Switsur VR (1989) Field measurements of photosynthesis, stomatal conductance, leaf nitrogen and δ13C along altitudinal gradients in Scotland. Func Ecol 3: 117–122Google Scholar
  12. Gale J (1972) Availability of carbon dioxide for photosynthesis at high altitudes: theoretical considerations. Ecology 53: 494–497Google Scholar
  13. Hermann RK, Lavender DP (1990) Pseudotsuga menziesii (Mirb.) Franco. In: Silvics of North America, Vol. 1, Conifers. Burns RM and Honkala BH (ed.) USDA Forest Service, Washington D.C.Google Scholar
  14. Hubick KT, Farquhar GD, Shorter R (1986) Correlation between water-use efficiency and carbon isotope discrimination in diverse peanut (Arachis) germplasm. Aust J Plant Physiol 13: 803–816Google Scholar
  15. Jaquish BC (1990) Geographic variation in ten-year height growth of interior Douglas-fir in British Columbia. In proceedings of joint meeting of WFGA and IUFRO, Olympia, Washington. 2.144Google Scholar
  16. Jarvis PG, McNaughton KG, (1986) Stomatal control of transpiration: scaling up from leaf to region. Adv Ecol Res 15: 1–49Google Scholar
  17. Johnson RC, Bassett LM (1991) Carbon isotope discrimination and water-use efficiency in four cool-season grasses. Crop Sci 31: 157–162Google Scholar
  18. Johnson RC, Asay KH, Tieszen LL, Ehleringer JR, Jefferson PG (1990) Carbon isotope discrimination: potential in screening cool-season grasses for water-limited environments. Crop Sci 30: 338–343Google Scholar
  19. Körner Ch, Diemer M (1987) In situ photosynthetic responses to light, temperature and carbon dioxide in herbaceous plants from low and high altitude. Func Ecol 1: 179–194Google Scholar
  20. Körner Ch, Mayr R (1981) Stomatal behaviour in alpine plant communities between 600 and 2600 meters above sea level. In: Grace J, Ford ED, Jarvis PG (eds.). Plant and their atmospheric environment. Blackwell, Oxford, pp 205–218Google Scholar
  21. Körner Ch, Farquhar GD, Roksandic Z (1988) A global survey of carbon isotope discrimination in plants from high altitude. Oecologia 74: 623–632Google Scholar
  22. Körner Ch, Farquhar GD, Wong SC (1991) Carbon isotope discrimination by plants follows latitudinal and altitudinal trends. Oecologia 88: 30–40Google Scholar
  23. Kozlowski TT, Kramer PJ, Pallardy SG (1991) The Physiological ecology of woody plants. Academic Press Inc. pp 31–68Google Scholar
  24. Ledig FT, Perry TO (1967) Variation in photosynthesis and respiration among loblolly pine progenies. South Conf For Tree Improve, 9th, pp 120–128Google Scholar
  25. Li P, Adams WT (1989) Range-wide patterns of allozyme variation in Douglas-fir (Pseudotsuga menziesii). Can J For Res 19: 149–161Google Scholar
  26. Martin B, Thorstenson YR (1988) Stable carbon isotope composition (δ13C), water use efficiency, and biomass productivity of Lycopersicon esculentum, Lycopersicon pennelli, and F1 hybrid. Plant Physiol 88: 10915–10933Google Scholar
  27. Monson RK, Grant MC (1989) Experimental studies of ponderosa pine. III. Differences in photosynthesis, stomatal conductance, and water-use efficiency between two genetic lines. Am J Bot 76: 1041–1047Google Scholar
  28. Morecroft MD, Woodward FI (1990) Experimental investigations on the environmental determination of δ13C at different altitudes. J Exp Bot 41: 1303–1308Google Scholar
  29. O'Leary MH (1988) Carbon isotopes in photosynthesis. BioScience 38: 325–336Google Scholar
  30. Pojar J, Klinka K, Meidinger DV (1987) Biogeoclimatic ecosystem classification in British Columbia. For Ecol Manage 22: 119–154Google Scholar
  31. Rao CR (1973) Linear statistical inference and its application (2nd ed.), New York: John WileyGoogle Scholar
  32. Read JJ, Farquhar, GD (1991) Comparative studies in Nothofagus (Fagaceae). I. Leaf carbon isotope discrimination. Func Ecol 5: 684–695Google Scholar
  33. Read JJ, Johnson RC, Carver BF, Quarrie SA (1991a) Carbon isotope discrimination, gas exchange, and yield of spring wheat selected for abscisic acid content. Crop Sci 31: 139–146Google Scholar
  34. Read JJ, Johnson DA, Asay KH, Tieszen LL (1991b) Carbon isotope discrimination, gas exchange, and water-use efficiency in creasted wheatgrass clones. Crop Sci 31: 1203–1208Google Scholar
  35. Rehfeldt GE (1986) Development and verification of models of freezing tolerance for Douglas-fir populations in the Inland Northwest. USDA For Serv Intermountain Res Stn, Res Paper INT-369, 5 pGoogle Scholar
  36. Rehfeldt GE (1989) Ecological adaptations in Douglas-fir (Pseudotsuga menziesii var. glauca): a synthesis. For Ecol Manage 28: 203–215Google Scholar
  37. SAS Institute (1985) SAS user's guide. Vol 2, SAS Institute, Inc., Cary, NCGoogle Scholar
  38. Smith WK, Donahue RA (1991) Simulated influence of altitude on photosynthetic CO2 uptake potential in plants. Plant Cell Env 14: 133–136Google Scholar
  39. Stephan BR (1987) Differences in the resistance of Douglas-fir provenances to the wooly aphid Gilletteella cooleyi. Silvae Genet 36: 76–79Google Scholar
  40. Sternberg LSL, Mulkey SS, Wright SJ (1989) Ecological interpretation of leaf carbon isotope ratios: influence of respired carbon dioxide. Ecology 70: 1317–1324Google Scholar
  41. Vitousek PM, Matson PA, Turner DR (1988) Elevational and age gradients in Hawaiian montane rainforest: foliar and soil nutrients. Oecologia 77: 565–570Google Scholar
  42. Vitousek PM, Field CB, Matson PA (1990) Variation in foliar δ13C in Hawaiian Metrosideros polymorpha: a case of internal resistance? Oecologia 84: 362–370Google Scholar
  43. WeatherDisc Associates, Inc. (1990) World WeatherDiscTM. Version 2.0. Seattle, WashingtonGoogle Scholar
  44. Woodward FI (1986) Ecophysiological studies on the shrub Vaccinium myrtillus L. taken from a wide altitudinal range. Oecologia 70: 580–586Google Scholar
  45. Woodward FI, Bazzaz FA (1988) The response of stomatal density to CO2 partial pressure. J Exp Bot 39: 1771–1781Google Scholar
  46. Wright GC, Hubick KT, Farquhar GD (1988) Discrimination in carbon isotopes of leaves correlates with water-use efficiency of field-grown peanut cultivars. Aust J Plant Physiol 15: 815–825Google Scholar
  47. Zavarin E, Snajberk K (1973) Geographic variability of monoterpenes from cortex of Pseudotsuga menziesii. Pure Appl Chem 34: 411–433Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Jianwei Zhang
    • 1
  • John D. Marshall
    • 1
  • Barry C. Jaquish
    • 2
  1. 1.Department of Forest ResourcesUniversity of IdahoMoscowUSA
  2. 2.Kalamalka Research Station and Seed OrchardB.C. Forest ServiceVernonCanada

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