, Volume 42, Issue 2, pp 191–203

Location of the genes for 5S ribosomal RNA in Xenopus laevis

  • Mary Lou Pardue
  • Donald D. Brown
  • Max L. Birnstiel


In situ hybridization of 5S RNA and cRNA transcribed in vitro from Xenopus laevis 5S DNA shows that 5S DNA is localized at or near the telomere region of the long arm of many, if not all, of the X. laevis chromosomes. No 5S DNA is detected near the nucleolus organizer in the normal X. laevis chromosome complement, but in a X. laevis kidney cell line, 5S DNA is found at the distal end of the secondary constriction. The arrangement of 5S DNA in several types of interphase nuclei is described. — During the pairing stages of meiosis the telomeres of most or perhaps all of the chromosomes become closely associated so that the regions containing 5S DNA form a single cluster. This close association might be either a cause or a result of the presence of the similar sequences of 5S DNA on many telomeres. It suggests that the uniformity of 5S sequences on non-homologous chromosomes might be maintained by crossing-over between the chromosomes.


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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Mary Lou Pardue
    • 1
    • 2
  • Donald D. Brown
    • 1
    • 2
  • Max L. Birnstiel
    • 1
    • 2
    • 4
  1. 1.Institute of Animal GeneticsUniversity of EdinburghScotland
  2. 2.Carnegie Institute for EmbryologyBaltimoreUSA
  3. 3.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA
  4. 4.Institut für Molekularbiologie 2Universität ZürichZürichSchweiz

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