, Volume 43, Issue 6, pp 367–371 | Cite as

Isolation of human monocytes by ficoll density gradient centrifugation

  • F. Berthold
Original Articles


The separation of human blood monocytes by a two step density gradient centrifugation using Ficoll is reported. From 10 ml venous blood 0.5×106 monocytes could be harvested with a purity of 72 ± 11%. Cells characteristics typical of monocytes (positive α-naphthylesterase staining, phagocytosis of polyacrylic acid beads, antigen presenting, adherence on plastic surfaces) confirmed the identity and the intact function of these cells.

Key words

Monocytes Separation techniques 


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • F. Berthold
    • 1
  1. 1.Universitäts-KinderpoliklinikGießenFederal Republic of Germany

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