Identification of leukemia cell-derived inhibitory activity (LIA) in conditioned media from human myeloid leukemic cell line ML-2
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Summary
Conditioned media from the human myeloid leukemic cell line ML-2 contain a factor that inhibits the entry of normal CFU-GM into S phase of mitotic cycle as measured by the 3H-TdR suicide technique. This factor was detected in conditioned media prepared by incubating 5×106 ML-2 cells/ml or 1×106 ML-2 cells/ml in serum-free RPMI for 5 or 24 hours respectively, and was isolated by ultrafiltration through an XM300 Diaflo membrane followed by chromatography on Sepharose 6B. Ferritin, prepared from human placenta, had the same inhibitory effect on CFU-GM. Antibodies against human placental ferritin completely inactivated the inhibitory effect of both human placental ferritin and the factor released from ML-2 cells. The inhibitory activity produced by the cell-line ML-2 was considered as LIA (leukemia cell-derived inhibitory activity) earlier found in HL-60 cell line and AML and CML cells.
Key words
LIA Leukemia cell-derived inhibitory activity CM Conditioned medium Granulopoiesis CFU-GM Progenitor cells of granulocytes and macrophagesPreview
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References
- 1.Bognacki J, Broxmeyer HE, Lobue J (1981) Isolation and biochemical characterization of leukemia associated inhibitory activity that suppresses colony and cluster formation of cells. Biochim Biophys Acta 672: 176–190Google Scholar
- 2.Broxmeyer HE, Jacobsen N, Kurland J, Mendelsohn N, Moore MAS (1978) In vitro suppression of normal granulocytic stem cells by inhibitory activity derived from human leukemia cells. J Nat Cancer Inst 60: 497–511Google Scholar
- 3.Broxmeyer HE, Bognacki J, Dorner MH, de Sousa M (1981) Identification of leukemia-associated inhibitory activity as acidic isoferritin. A regulatory role for acidic isoferritin in the production of granulocytes and macrophages. J Exp Med 153: 1426–1444Google Scholar
- 4.Broxmeyer HE (1982) Relationship of cell-cycle expression of Ia-like antigenic determinants on normal and leukemic human granulocyte-macrophage progenitor cells to regulation in vitro by acidic iso-ferritins. J Clin Invest 69: 632–642Google Scholar
- 5.Broxmeyer HE, Bognacki J, Ralph P, Dorner MH, Lu L, Castro-Malaspina H (1982) Monocyte-macrophage derived acidic isoferritins: normal feedback regulators of granulocyte-macrophage progenitor cells. Blood 60: 595–607Google Scholar
- 6.Broxmeyer HE (1983) Acid isoferritin inhibitory activity. Blood 62: 702–703Google Scholar
- 7.Broxmeyer HE, Gentile P, Bognacki J, Ralph P (1983) Lactoferrin, transferrin and acidic isoferritins: regulatory molecules with potential therapeutic value in leukemia. Blood Cells 9: 83–105Google Scholar
- 8.Drysdale JW, Munro HN (1965) Small-scale isolation of ferritin for the assay of the incorporation of 14C-labelled amino acids. Biochem J 95: 851–858Google Scholar
- 9.Eckersall D, Conner JG (1984) The prevention of distortion in ultrathin-layer polyacrylamide gel isoelectric focusing. Anal Biochem 138: 52–58Google Scholar
- 10.Minowada J, Sagawa K, Trowbrodge IS, Kung PD, Goldstein G (1982) Markerprofiles of 55 human leukemia-lymphoma cell lines. In: Kaplan HS, Rosenberg SA (eds) Malignant lymphomas. Academic Press, New York, p 53Google Scholar
- 11.Olofsson T, Olsson I (1980) Suppression of normal granulopoiesis in vitro by a leukemia associated inhibitor (LAI) derived from human prolyelotic cell line (HL-60). Leuk Res 4: 437–447Google Scholar
- 12.Olofsson T, Olsson I (1980) Biochemical characterization of a leukemia-associated inhibitor (LAI) suppressing normal granulopoiesis in vitro. Blood 55: 983–991Google Scholar