Cell and Tissue Research

, Volume 275, Issue 2, pp 299–308 | Cite as

Octopamine-like immunoreactive neurones in locust genital abdominal ganglia

  • Paul A. Stevenson
  • Hans-Joachim Pflüger
  • Manfred Eckert
  • Jürgen Rapus


Using a well characterized anti-serum, the distribution of octopamine-like immunoreactive neurones is described in the locust seventh abdominal (A7) and terminal ganglia (TG), which are associated with genital organs. Apart from 4 paired ventral somata occasionally observed in the TG, all labelled cells could be identified as efferent dorsal- and ventral unpaired median (DUM/VUM) neurones by virtue of the characteristic large size and position of their somata, projections of their primary neurites in DUM-cell tracts, and bifurcating axons which arise from dorsal T-junctions and enter peripheral nerves. For the examined ganglia our data indicate that the whole population of efferent DUM and VUM-cells, defined here as progeny of the segment specific unpaired median neuroblast with peripheral axons, are octopaminergic, and that equal numbers of these cells occur in both sexes: 8 in A7 and 11 in TG. Sex-specific differences are probably restricted to the axonal projections of 5 octopamine-like immunoreactive DUM-somata in A7, and 5 in TG, which in females project into their segment specific sternal nerves, but in males into the genital nerve of the TG. Numerous intersegmentally projecting octopamine-like immunoreactive fibres traverse both ganglia. The majority probably stem from previously described octopamine-like immunoreactive neurones in the thoracic and suboesophageal ganglia.

Key words

Immunocytochemistry Amines, biogenic DUM neurones Sexual dimorphism Locusta migratoria (Insecta) 


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  1. Agricola H, Hetel W, Penzlin H (1988) Octopamin — Neurotransmitter, Neuromodulator und Neurohormon. Zool Jb Physiol 92:1–45Google Scholar
  2. Agricola H, Schildberger K, Schmidt A, Naumann W, Reißmann S, Huber F, Penzlin H (1992) Imunocytochemical distribution of allatostatin in the nervous system of the cockroach Periplaneta americana. In: Elsner N, Richter DW (eds) Rhythmogenesis in neurones and networks. Proceedings of the 20th Göttingen Neurobiology Conference. Thieme, Stuttgart New York, p 494Google Scholar
  3. Bate M, Goodman CS, Spitzer NC (1981) Embryonic development of identified neurones: Segment specific differences in the cell homologues. J Neurosci 1:103–106Google Scholar
  4. Bräunig P (1991) Suboesophageal DUM neurons innervate the principal neuropiles of the locust brain. Philos Trans R Soc Lond [Biol] 332:221–240Google Scholar
  5. Doc CD, Goodman CS (1985) Early events in insect neurogenesis I. Development and segmental differences in the pattern of neuronal precursor cells. Dev Biol 111:193–205Google Scholar
  6. Eckert M, Varanka I, Benedecky I (1989) Proctolinergic innervation of the locust oviduct. Zool Jb Physiol 93:471–479Google Scholar
  7. Eckert M, Rapus J, Nürnberger A, Penzlin H (1992) A new specific antibody reveals octopamine-like immunoreactivity in cockroach ventral nerve cord. J Comp Neurol 322:1–15Google Scholar
  8. Evans PD (1985) Octopamine. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology. Pergamon Press, Oxford, pp 499–530Google Scholar
  9. Evans PD, O'Shea M (1978) The identification of an octopaminergic neurone and the modulation of a myogenic rhythm in the locust. J Exp Biol 73:235–260Google Scholar
  10. Falck B, Hillap N-A, Thieme G, Torp A (1962) Fluorescence of catacholamines and related compounds condensed with formaldehyde. J Histochem Cytochem 10:348–354Google Scholar
  11. Ferber M, Pflügr H-J (1990) Bilaterally projecting neurones in pregenital abdominal ganglia of the locust: anatomy and periheral targets. J Comp Neurol 302:447–460Google Scholar
  12. Ferber M, Pflüger H-J (1992) An identified dorsal unpaired median neurone and bilaterally projecting neurones exhibiting bovine pancreatic polypeptide-like/FMRFamide-like immunoreactivity in abdominal ganglia of the migratory locust. Cell Tissue Res 267:85–98Google Scholar
  13. Giebultowicz JM, Truman JW (1984) Sexual differentiation in the terminal ganglion of the moth Manduca sexta: role of sex-specific cell death. J Comp Neurol 226:87–95Google Scholar
  14. Goodman CS (1982) Embryonic development of identified neurones in the grasshopper. In: Spitzer NC (ed) Neuronal development. Plenum Press, New York, pp 171–212Google Scholar
  15. Heckmann R (1988) Die Funktion der Innervierung der Genital-kammer von Acheta domesticus (L). Diplomarbeit, Universität KonstanzGoogle Scholar
  16. Kalogianni E, Pflüger H-J (1992) The identification of motor and unpaired median neurones innervating the locust oviduct. J Exp Biol 168:177–198Google Scholar
  17. Kimura T, Yasuyama K, Yamaguchi T (1989) Proctolinergic innervation of the accessory glands in male crickets (Gryllus bimaculatus): detection of proctolin and some pharmacological properties of myogenically and neurogenically evoked contractions. J Insect Physiol 35:251–264Google Scholar
  18. Kiss T, Varanka I, Benedeczky I (1984) Neuromuscular transmission in the visceral muscle of locust oviduct. Neuroscience 12:309–322Google Scholar
  19. Klemm N (1976) Histochemistry of putative transmitter substances in the insect brain. Prog Neurobiol 7:99–169Google Scholar
  20. Konings PNM, Vullings HGB, Geffard M, Buijs RM, Diederen JHB, Jansen WF (1988) Immunocytochemical demonstration of octopamine-immunoreactive cells in the nervous system of Locusta migratoria and Schistocerca gregaria. Cell Tissue Res 251:371–379Google Scholar
  21. Lange AB, Orchad I (1984) Dorsal unpaired median neurons and ventral bilaterally paired neurones, project to a visceral muscle in an insect. J Neurobiol 15:441–453Google Scholar
  22. Lange AB, Orchard I (1986) Ventral neurons in an abdominal ganglion of the locust Locusta migratoria, with properties similar to dorsal unpaired median neurons. Can J Zool 64:264–267Google Scholar
  23. Nürnberger A, Rapus J, Eckert M, Penzlin H (1990) Taurine-, octopamine-, and proctolin-like immunoreactivity in neurons of the American cockroach Periplaneta americana L. In: Elsner N, Roth G (eds) Brain — perception cognition. Proceedings of the 18th Göttingen Neurobiology Conference. Thieme, Stuttgart, p 490Google Scholar
  24. O'Shea M, Adams ME (1981) Pentapeptide (proctolin) associated with an identified neuron. Science 213:567–569Google Scholar
  25. Orchard I, Lange AB (1985) Evidence for octopaminergic modulation of an insect visceral muscle. J Neurobiol 16:171–181Google Scholar
  26. Orchard I, Lange AB, Cook H, Ramirez J-M (1989) A subpopulation of dorsal unpaired median neurons in the blood-feeding insect Rhodnius prolixus displays serotonin-like immunoreactivity. J Comp Neurol 289:118–128Google Scholar
  27. Pflüger H-J, Watson ADH (1988) Structure and distribution of dorsal unpaired median (DUM) neurones in the abdominal nerve cord of male and female locusts. J Comp Neurol 268:329–345Google Scholar
  28. Pflüger H-J, Witten JL, Levine RB (1993) Fate of abdominal ventral unpaired median (VUM) neurones during metamorphosis of the hawkmoth, Manduca sexta. J Comp Neurol (in press)Google Scholar
  29. Roonwal ML (1937) Studies on the embryology of the African migratory locust, Locusta migratoria migratorioides Reiche and Frm. Philos Trans R Soc [Biol] 227:175–244Google Scholar
  30. Seabrook WD (1968) The innervation of the terminal abdominal segments (VIII–XI) of the desert locust, Schistocerca gregaria. Canadian Entomologist 100:693–715Google Scholar
  31. Spörhase-Eichmann U, Vullings HGB, Buijs RM, Hörner M (1992) Octopamine-immunoreactive neurones in the central nervous system of the cricket Gryllus bimaculatus. Cell Tissue Res 268:287–304Google Scholar
  32. Stevenson PA, Pflüger H-J, Eckert M, Rapus J (1992a) Octopamine immunoreactive cell population in locust thoracic-abdominal nervous system. J Comp Neurol 315:382–397Google Scholar
  33. Stevenson PA, Ferber M, Pflüger H-J (1992b) Co-localisation of octopamine- & FMRFamide/BPP-like immunoreactivity in an identified locust DUM-neurone. In: Elsner N, Richter DW (eds) Rhythmogenesis in neurones and networks. Proceedings of the 20th Göttingen Neurobiology Conference. Thieme, Stuttgart New York p 479Google Scholar
  34. Stoya GM, Agricola H, Eckert M, Penzlin H (1989) Investigations on the innervation of the oviduct muscle of the cockroach, Periplaneta americana (L). Zool Jb Physiol 93:75–86Google Scholar
  35. Thompson KJ (1986) Oviposition digging in the grasshopper 1. Functional anatomy and the motor programme. J Exp Biol 122:387–411Google Scholar
  36. Thompson KJ, Siegler MVS (1989) Properties of the small dorsal unpaired median (DUM) neurons of the grasshopper. Soc Neurosci Abstr 15:1296Google Scholar
  37. Thompson KJ, Siegler MVS (1991) Anatomy and physiology of spiking local and intersegmental interneurons in the median neuroblast lineage of the grasshopper. J Comp Neurol 305:659–675Google Scholar
  38. Thorn RS, Truman JW (1989) Sex-specific neuronal respecification during the metamorphosis of the tobacco hornworm moth Manduca sexta. J Comp Neurol 284:489–503Google Scholar
  39. Tyrer NM, Gregory GE (1982) A guide to the neuroanatomy of locust suboesophageal and thoracic ganglia. Philos Trans R Soc Lond [Biol] 297:91–123Google Scholar
  40. Watson ADH (1984) The dorsal unpaired median neurons of the locust metathoracic ganglion: neuronal structure and diversity, and synaptic distribution. J Neurocytol 13:303–327Google Scholar
  41. Whim MD, Evans PD (1989) Age-dependence of octopaminergic modulation of flight muscle in the locust. J Comp Physiol [A] 165:125–137Google Scholar
  42. Yamaguchi T, Kushiro N, Waki T (1985) Sexual dimorphism of the terminal abdominal ganglion of the cricket. Naturwis-senschaften 72:153–154Google Scholar
  43. Yasuyama K, Kumura T, Yamaguchi T (1988) Musculature and innervation of the internal reproductive organs in the male cricket, with special reference to the projection of unpaired median neurones of the terminal abdominal ganglion. Zool Sci 5:767–780Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Paul A. Stevenson
    • 1
  • Hans-Joachim Pflüger
    • 1
  • Manfred Eckert
    • 2
  • Jürgen Rapus
    • 2
  1. 1.Institut für NeurobiologieFreie Universität BerlinBerlinGermany
  2. 2.Institut für Allgemeine Zoologie und TierphysiologieFriedrich-Schiller Universität JenaJenaGermany

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