Cell and Tissue Research

, Volume 268, Issue 3, pp 471–478

Receptor-mediated and adsorptive endocytosis by male germ cells of different mammalian species

  • Dominique Segretain
  • Monique Egloff
  • Nadine Gérard
  • Charles Pineau
  • Bernard Jégou
Article

Summary

The routes for adsorptive and receptor-mediated endocytosis were studied in vivo after microinjection of tracers into the lumen of the seminiferous tubules, and in vitro in isolated germ cells of different mammals. Cationic ferritin was located on the plasma membrane, in vesicles, in tubules, in multivesicular bodies and in lysosome-like granules of mouse spermatocytes. In these cells the number of multivesicular bodies varied during spermatogenesis. Spermatids and to a lesser extent residual bodies also performed adsorptive endocytosis. In the rat and monkey (Macaca fascicularis) diferric transferrin was specifically taken up by germ cells via receptor-mediated endocytosis. The labelling was observed subsequently in membrane pits, vesicles, endosome-like bodies and pale multivesicular bodies. A progressive decrease in the frequency of the labelling of the germ cells by transferrin-gold particles was observed from spermatogonia to spermatocytes and to early spermatids, which could indicate that iron is particularly required by germ cells during the mitotic and meiotic processes. Adsorptive and receptor-mediated endocytosis therefore occurs in all classes of germ cells. These endocytic processes are most probably required for germ cell division, differentiation and metabolism.

Key words

Testis Endocytosis Spermatogenesis Spermatogonia Spermatocytes Spermatids Transferrin Ferritin Sertoli cells Mouse Rat Monkey, Macaca fascicularis (Primates) 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Dominique Segretain
    • 1
  • Monique Egloff
    • 2
  • Nadine Gérard
    • 3
  • Charles Pineau
    • 3
  • Bernard Jégou
    • 3
  1. 1.Laboratoire d'Histologie-EmbryologieCHU Paris OuestParisFrance
  2. 2.Faculté de MédecineLaboratoire Hospitalo-UniversitaireParisFrance
  3. 3.Groupe d'Etude de la Reproduction chez le Mâle INSERM, CJF 91-04Université de Rennes 1Rennes CedexFrance

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