Cell and Tissue Research

, Volume 274, Issue 2, pp 267–277

Immunohistochemical localization of GABAA receptors in the scotopic pathway of the cat retina

  • Ulrike Grünert
  • Thomas E. Hughes
Article

Abstract

The distribution of GABAA receptors in the inner plexiform layer of cat retina was studied using monoclonal antibodies against the β2/β3 subunits. A dense band of receptor labeling was found in the inner region of the inner plexiform layer where the rod bipolar axons terminate. Three forms of evidence indicate that the GABAA receptor labeling is on the indoleamine-accumulating, GABAergic amacrine cell that is synaptically interconnected with the rod bipolar cell terminal. (1) Electron microscopy showed that the anti-GABAA receptor antibody (62-3G1) labeled profiles that were postsynaptic to rod bipolar axons and made reciprocal synapses. (2) Indoleamine uptake (and the subsequent autofluorescence) combined with GABAA receptor immunohistochemistry showed co-localization of the two markers in half of the receptor-positive amacrine cells. (3) Double labeling demonstrated that half of the receptor-positive somata also contained GABA. These results indicate that a GABAergic amacrine cell interconnected with the rod bipolar cell, most likely the so-called A17 amacrine cell, itself bears GABAA receptors.

Key words

Retina, mammalian GABAA receptors, β2/β3 subunits Amacrine cells Bipolar cells Rod pathway Cat 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Ulrike Grünert
    • 1
  • Thomas E. Hughes
    • 2
  1. 1.Max-Planck-Institut für HirnforschungFrankfurt am MainGermany
  2. 2.Department of Neurosciences, 0608University of Califorinia, San DiegoLa JollaUSA
  3. 3.Department of Ophthalmology and Visual ScienceYale University School of MedicineNew HavenUSA

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