Cell and Tissue Research

, Volume 267, Issue 1, pp 193–197 | Cite as

Immunohistochemical localization of transthyretin in glomerular peripolar cells of newborn sheep

  • Catherine A. Hollywell
  • Anthony Jaworowski
  • Cassandra Thumwood
  • Daine Alcorn
  • Graeme B. Ryan
Article
Received:
Accepted:

Summary

Purified transthyretin has been isolated from sheep serum. Antiserum raised against this protein has been used with an indirect immunoperoxidase histochemical technique to identify transthyretin in newborn lamb kidney tissue. Transthyretin was found in proximal tubule cells and in glomerular peripolar cells. Preabsorption studies using purified transthyretin protein indicate that the immunoreactivity of the antiserum is specific to transthyretin.

Key words

Transthyretin Kidney Peripolar cells Sheep, newborn 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alcorn D, Cheshire GR, Coghlan JP, Ryan GB (1984) Peripolar cell hypertrophy in the renal juxtaglomerular region of newborn sheep. Cell Tissue Res 236:197–202Google Scholar
  2. Dickson PW, Howlett GJ, Schreiber G (1985) Rat transthyretin (prealbumin) molecular cloning, nucleotide sequence and gene expression in liver and brain. J Biol Chem 260:8214–8219Google Scholar
  3. Dussault JH, Ruel J (1987) Thyroid hormones and brain development. Annu Rev Physiol 49:321–334Google Scholar
  4. Gall JAM, Alcorn D, Butkus A, Coghlan JP, Ryan GB (1986) Distribution of glomerular peripolar cells in different mammalian species. Cell Tissue Res 244:203–208Google Scholar
  5. Gardiner DS, Lindop GBM (1985) The granular peripolar cell of the human glomerulus: a new component of the juxtaglomerular apparatus? Histopath 9:675–685Google Scholar
  6. Gray HDA, Gray ES, Horne HW (1985) Sites of prealbumin production in the huma fetus using the indirect immunoperoxidase technique. Vichows Arch [A] 406:463–473Google Scholar
  7. Hanner RH, Ryan GB (1980) Ultrastructure of the renal juxtaglomerular complex and peripolar cells in the axolotl (Ambystoma mexicanum) and toad (Bufo marinus). J Anat 130:445–455Google Scholar
  8. Hill PA, Coghlan JP, Scoggins BA, Ryan GB (1983) Ultrastructural changes in the sheep renal juxtaglomerular apparatus in response to sodium depletion or loading. Pathol 15:463–473Google Scholar
  9. Jacobsson B (1989) In situ localization of transthyretin-mRNA in the adult human liver, choroid plexus and pancreatic islets and in endocrine tumours of the pancreas and gut. Histochem 91:299–304Google Scholar
  10. Kanai M, Raz A, Goodman DS (1968) Retinol-binding protein the transport protein for vitamin A in human plasma. J Clin Invest 47:2025–2044Google Scholar
  11. Kato M, Kato K, Goodman DS (1984) Immunocytochemical studies on the localization of plasma and of cellular retinol-binding proteins and of transthyretin (prealbumin) in rat liver and kidney. J Cell Biol 98:1696–1704Google Scholar
  12. Katoh M, Kanai M, Kameko M, Ohno S, Fujii Y, Nagata T (1982) Localization of retinol-binding protein and prealbumin in the human kidney with an unlabeled enzyme immunohistochemical method. Acta Histochem Cytochem 15:68–75Google Scholar
  13. Kleinman LI, Reuter JH (1973) Maturation of glomerular blood flow distribution in the newborn dog. J Physiol 228:91–103Google Scholar
  14. Laurell CB (1965) Antigen-antibody crossed electrophoresis. Anal Biochem 10:358–361Google Scholar
  15. Mendel CM, Cavalieri RR, Gavin LA, Pettersson T, Inouye M (1989) Thyroxine transport and distribution in Nagase analbuminemic rats. J Clin Invest 83:143–148Google Scholar
  16. Morild I, Christensen JA, Mikeler E, Bohle A (1988) Peripolar cells in the avian kidney. Virchows Archiv (A) 412:471–477Google Scholar
  17. Navab M, Mallia AK, Kanda Y, Goodman DS (1977) Rat plasma prealbumin. Isolation and partial characterization. J Biol Chem 252:5100–5106Google Scholar
  18. Rask L, Peterson PA, Nilsson SF (1971) The subunit structure of human thyroxine-binding prealbumin. J Biol Chem 246:6087–6097Google Scholar
  19. Ryan GB, Coghlan JP, Scoggins BA (1979) The granulated peripolar epithelial cell: a potential secretory component of the renal juxtaglomerular complex. Nature 277:655–656Google Scholar
  20. Schaeverbeke J, Cheignon M (1980) Differentiation of glomerular filter and tubular reabsorption apparatus during foetal development of the rat kidney. J Embryol Exp Morphol 58:157–175Google Scholar
  21. Souster LP, Emery JL (1980) The sizes of renal glomeruli in foetuses and infants. J Anat 130:595–602Google Scholar
  22. Taugner R, Hackenthal E (1989) The juxtaglomerular apparatus, 1st ed. Springer, Berlin Heidelberg New York, pp 50–52Google Scholar
  23. Trahair JF, Ryan GB (1988) Immunohistochemical identification of plasma proteins in cytoplasmic granules of peripolar cells of the sheep. J Anat 160:109–115Google Scholar
  24. Trahair JF, Gall JAM, Alcorn D, Coghlan JP, Fernley R, Penschow J, Grattage LP, Johnston CI, Ryan GB (1989) Immunohistochemical study of peripolar cells of the sheep. J Anat 162:125–132Google Scholar
  25. Wakasugi S, Maeda S, Shimada K (1986) Structure and expression of the mouse prealbumin gene. J Biochem 100:49–58Google Scholar
  26. Wilson JG, Roth CB, Warkany J (1953) An analysis of the syndrome of malformations induced by maternal vitamin A deficiency. Effects of restoration of vitamin A at various times during gestation. Am J Anat 92:189–217Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Catherine A. Hollywell
    • 1
  • Anthony Jaworowski
    • 2
  • Cassandra Thumwood
    • 1
  • Daine Alcorn
    • 1
  • Graeme B. Ryan
    • 1
  1. 1.Department of AnatomyUniversity of MelbourneParkvilleAustralia
  2. 2.Department of BiochemistryUniversity of MelbourneParkvilleAustralia

Personalised recommendations