Cell and Tissue Research

, Volume 267, Issue 1, pp 99–104

Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes

  • Miranda D. Grounds
  • Kerryn L. Garrett
  • May C. Lai
  • Woodring E. Wright
  • Manfred W. Beilharz
Article

DOI: 10.1007/BF00318695

Cite this article as:
Grounds, M.D., Garrett, K.L., Lai, M.C. et al. Cell Tissue Res (1992) 267: 99. doi:10.1007/BF00318695

Summary

The activation of mononuclear muscle precursor cells after crush injury to mouse tibialis anterior muscles was monitored in vivo by in situ hybridization with MyoD1 and myogenin probes. These genes are early markers of skeletal muscle differentiation and have been extensively studied in vitro. The role in vivo of these regulatory proteins during myogenesis of mature muscle has not been studied previously. MyoD1 and myogenin mRNA were present in occasional mononuclear cells of uninjured muscle. Increased MyoD1 and myogenin mRNA sequences in mononuclear cells were detected as early as 6 h after injury, peaked between 24 and 48 h, and thereafter declined to pre-injury levels at about 8 days. The mRNAs were detected in mononuclear cells throughout the muscle, with the majority of cells located some distance from the site of crush injury. The presence of MyoD1 and myogenin mRNA at 6 to 48 h indicates that transcription of these genes is occurring at the same time as replication of muscle precursor cells in vivo. At no time were significant levels of mRNA for these genes detected in myotubes. MyoD1 and myogenin provide precise markers for the very early identification and study of mononuclear skeletal muscle precusor cells in muscle regenerating in vivo.

Key words

Skeletal muscle Myogenesis Muscle regeneration MyoD1 Myogenin In situ hybridisation Mouse (Swiss SJL/J) 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Miranda D. Grounds
    • 1
  • Kerryn L. Garrett
    • 2
  • May C. Lai
    • 2
  • Woodring E. Wright
    • 3
  • Manfred W. Beilharz
    • 2
  1. 1.Department of Pathology, Queen Elizabeth II Medical CentreThe University of Western AustraliaNedlandsAustralia
  2. 2.Department of Microbiology, Queen Elizabeth II Medical CentreThe University of Western AustraliaNedlandsAustralia
  3. 3.Department of Cell Biology and AnatomyThe University of Texas Southwestern Medical Center at DallasDallasUSA

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