Current Genetics

, Volume 21, Issue 1, pp 83–84 | Cite as

One-step transformation of yeast in stationary phase

  • Dz-Chi Chen
  • Bei-Chang Yang
  • Tsong-Teh Kuo
Short Communications


A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

Key words

Yeast Transformation Thio compound Stationary phase 


  1. Brzobohatý B, Kováč L (1986) J Gen Microbiol 132:3089–3093Google Scholar
  2. Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933Google Scholar
  3. Ito H, Fukuda Y, Murata K, Kimura A (1983a) J Bacteriol 153:163–168Google Scholar
  4. Ito H, Murata K, Kimura A (1983b) Agric Biol Chem 47:1691–1692Google Scholar
  5. Ito H, Murata K, Kimura A (1984) Agric Biol Chem 48:341–347Google Scholar
  6. Jones E (1976) Genetics 85:23–30Google Scholar
  7. Kadohda D, Abe N, Matsui M, Hayashibe M (1976) Plant Cell Physiol 17:909–919Google Scholar
  8. Kitamura K (1982) Agric Biol Chem 46:2093–2099Google Scholar
  9. Klebe RJ, Harriss JV, Sharp JD, Douglas MG (1983) Gene 25:223–241Google Scholar
  10. Meilhoc E, Masson J-M, Teissié J (1990) Bio/Technology 8:233–227Google Scholar
  11. Pastor FIJ, Herrero E, Sentandreu R (1982) Arch Microbiol 22:249–258Google Scholar
  12. Sikorski RS, Hieter P (1989) Genetics 122:19–27Google Scholar
  13. Sterns T, Hong M, Bostein D (1990) Methods Enzymol 185:280–297Google Scholar
  14. Zlotnik H, Fernadez MP, Bower B, Cabib E (1984) J Bacteriol 159:1018–1026Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Dz-Chi Chen
    • 1
    • 3
  • Bei-Chang Yang
    • 3
  • Tsong-Teh Kuo
    • 1
    • 2
    • 3
  1. 1.Graduate Institute of BotanyNational Taiwan UniversityTaipeiRepublic of China
  2. 2.Institute of BotanyAcademia SinicaTaipeiRepublic of China
  3. 3.Institute of Molecular BiologyAcademia SinicaTaipeiRepublic of China

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