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Cell and Tissue Research

, Volume 260, Issue 2, pp 235–247 | Cite as

Immunoreactive atrial natriuretic peptide in the fish heart and blood plasma examined by electron microscopy, immunohistochemistry and radioimmunoassay

  • Haruko Uemura
  • Mitsuhide Naruse
  • Tohru Hirohama
  • Sumio Nakamura
  • Yoko Kasuya
  • Tomoji Aoto
Article

Summary

The immunoreactivity of atrial natriuretic peptide and ultrastructure of cardiocytes were examined in 5 species each of freshwater and seawater teleosts, as well as in 2 species each of elasmobranchs and cyclostomes. Immunoreactivity was strong in the atria of Cyprinus carpio, Anguilla japonica and Conger myriaster, rather weak in atria of Channa maculata, Lepomis macrochirus, Salmo gairdneri, Oplegnathus fasciatus and Eptatretus burgeri, very weak in atria of Pagrus major, Trachurus japonicus and Triakis scyllia, and not detectable in atria of Hexagrammos otakii, Narke japonica and Lampetra japonica. The immunoreactivity of the atrial cardiocytes was generally stronger in freshwater than seawater fish. Ventricular immunoreactivity was detected only in 7 species, always being weaker than that observed in the atrium. Ultrastructurally, however, secretory granules were found in atria and ventricles of all species examined, being more frequent in the former than the latter. By radioimmunoassay, immunoreactive ANP was detected in the extracts of blood plasma and both atrial and ventricular tissues of all species examined. There were no statistically significant differences in the values between freshwater and seawater species.

Key words

Atrial natriuretic peptide Heart Freshwater fish Seawater fish Immunohistochemistry Ultrastructure Radioimmunoassay Cyprinus carpio (Teleostei) Narke japonica (Elasmobranchii) Eptatretus burgeri (Cyclostomata) 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Haruko Uemura
    • 1
  • Mitsuhide Naruse
    • 2
  • Tohru Hirohama
    • 1
  • Sumio Nakamura
    • 1
  • Yoko Kasuya
    • 1
  • Tomoji Aoto
    • 1
  1. 1.Biological LaboratoryKanagawa Dental CollegeYokosuka-shiJapan
  2. 2.Department of MedicineTokyo Women's Medical CollegeTokyoJapan

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