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Current Genetics

, Volume 18, Issue 3, pp 239–243 | Cite as

Structure and expression of tomato mitochondrial genes coding for tRNACys (GCA), tRNAAsn (GUU) and tRNATyr (GUA): A native tRNACys gene is present in dicot plants but absent in monocot plants

  • Shinichi Izuchi
  • Tohru Terachi
  • Masahiro Sakamoto
  • Tetsuo Mikami
  • Mamoru Sugita
Original Articles

Summary

The nucleotide sequences of tRNAAsn (GUU) and tRNATyr (GUA) genes from tomato mitochondria and their flanking regions have been determined. The tomato mitochondrial tRNAAsn gene is located 2.1 kb downstream from the tRNACys gene reported previously (Izuchi and Sugita 1989) and shows a nearly complete identity with the corresponding chloroplast gene. The tRNATyr gene, which shows only 73% homology with the corresponding chloroplast gene, has to be considered a “native” mitochondrial tRNA gene and is 535 bp from the “chloroplast-like” tRNAAsn gene on the same strand. Northern hybridization analysis revealed that the three tRNA genes are transcribed in tomato mitochondria. Southern hybridization analysis of tomato, sugar beet, rice and wheat mitochondrial DNAs, with oligonucleotide probes for mitochondrial or chloroplast tRNA genes, demonstrated that the mitochondrial tRNACys gene found in tomato is present in dicot plants but not in monocots. On the other hand, a chloroplast-like tRNACys gene exists in monocot plants.

Key words

Mitochondria tRNA genes Tomato Transcription 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Shinichi Izuchi
    • 1
  • Tohru Terachi
    • 2
  • Masahiro Sakamoto
    • 3
  • Tetsuo Mikami
    • 4
  • Mamoru Sugita
    • 5
  1. 1.Department of Botany, Faculty of ScienceHokkaido UniversitySapporoJapan
  2. 2.Institute for National Land Utilization and DevelopmentKyoto Sangyo UniversityKyotoJapan
  3. 3.Plant Biotechnology DepartmentLife Science Laboratory, Mitsui Toatsu Chemicals, IncMobaraJapan
  4. 4.Laboratory of Industrial Crops, Faculty of AgricultureHokkaido UniversitySapporoJapan
  5. 5.Center for Gene ResearchNagoya UniversityNagoyaJapan

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