Current Genetics

, Volume 18, Issue 3, pp 175–179

Role of the CDC8 gene in the repair of single strand breaks in DNA of the yeast Saccharomyces cerevisiae

  • H. Baranowska
  • D. Zaborowska
  • W. J. Jachymczyk
  • J. Żuk
Original Articles
  • 29 Downloads

Summary

It has been found that the repair of single strand breaks is defective in the DNA replication mutants cdc8-1 and cdc8-3 of Saccharomyces cerevisiae both in permissive (23°C) and restrictive conditions (36°C). In permissive conditions we observed a significant delay in single strand break repair in a diploid strain HB7 (cdc8-1/cdc8-1), as compared with the wild-type strain. Under restrictive conditions no repair was observed, but rather degradation of MMS-damaged DNA occurred. It has been also found that the repair of single strand breaks in yeast is inhibited by cycloheximide but not by hydroxyurea.

Key words

CDC8 gene DNA repair 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • H. Baranowska
    • 1
  • D. Zaborowska
    • 1
  • W. J. Jachymczyk
    • 1
  • J. Żuk
    • 1
  1. 1.Institute of Biochemistry and BiophysicsPolish Academy of SciencesWarsawPoland

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