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Current Genetics

, Volume 18, Issue 4, pp 293–301 | Cite as

Functional analysis of the sporulation-specific SPR6 gene of Saccharomyces cerevisiae

  • L. A. Kallal
  • M. Bhattacharyya
  • S. N. Grove
  • R. F. Iannacone
  • T. A. Pugh
  • D. A. Primerano
  • M. J. Clancy
Original Articles

Summary

The SPR6 gene of Saccharomyces cerevisiae encodes a moderately abundant RNA that is present at high levels only during sporulation. The gene contains a long open reading frame that could encode a hydrophilic protein approximately 21 kDa in size. This protein is probably produced by the yeast, because the lacZ gene of Escherichia coli is expressed during sporulation when fused to SPR6 in the expected reading frame. SPR6 is inessential for sporulation; mutants that lack SPR6 activity sporulate normally and produce viable ascospores. Nonetheless, the SPR6 gene encodes a function that is relevant to sporulating cells; the wild-type allele can enhance sporulation in strains that are defective for several SPR functions. SPR6 is located on chromosome V, 14.4 centimorgans centromere-distal to MET6.

Key words

Saccharomyces cerevisiae Sporulation Inessential genes Genome organization 

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References

  1. Atecheson C, DiDominico B, Frackman S, Esposito RE (1987) Proc Natl Acad Sci USA 84:8035–8039Google Scholar
  2. Aviv H, Leder P (1972) Proc Natl Acad Sci USA 69:1408–1412Google Scholar
  3. Boeke JD, Garfinkel DJ, Styles CA, Fink JR (1985) Cell 40:491–500Google Scholar
  4. Bradford M (1976) Anal Biochem 72:248–254Google Scholar
  5. Breter H-J, Ferguson J, Peterson TA, Reed SI (1983) Mol Cell Biol 3:881–891Google Scholar
  6. Broach JR, Strathern JN, Hicks JB (1979) Gene 8:121–133Google Scholar
  7. Casabadan MJ, Martinez-Arias A, Shapira SK, Chou J (1983) Methods Enzymol 100:293–308Google Scholar
  8. Clancy MJ, Buten-Magee B, Straight DJ, Kennedy A, Partridge RM, Magee PT (1983) Proc Natl Acad Sci USA 80:3000–3004Google Scholar
  9. Coleman KG, Steensma HY, Kaback DB, Pringle JR (1986) Mol Cell Biol 6:4516–4525Google Scholar
  10. Conover WJ (1981) Practical Nonparametric Statistics, 2nd edn. John Wiley and Sons, New York, pp 143–153Google Scholar
  11. Davis RW, Botstein D, Roth JR (1980) A manual for genetic engineering: Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  12. Engel JD, Dodgson JB (1981) Proc Natl Acad Sci USA 78:2856–2860Google Scholar
  13. Esposito RE, Klapholz S (1981) In: Stathern JN, Jones EW, Broach JR (eds) Molecular biology of the yeast Sacchromyces: Life cycle and inheritance. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 211–287Google Scholar
  14. Garber AT, Segall J (1986) Mol Cell Biol 6:4478–4485Google Scholar
  15. Goebl M, Petes TD (1986) Cell 46:983–992Google Scholar
  16. Gottlin-Ninfa E, Kaback DB (1986) Mol Cell Biol 6:2185–2197Google Scholar
  17. Guarante L (1987) Annu Rev Genet 21:425–452Google Scholar
  18. Hanahan D (1983) J Mol Biol 166:557–580Google Scholar
  19. Holaway BL, Lehman DJ, Primerano DA, Magee PT, Clancy MJ (1985) Curr Genet 10:163–169Google Scholar
  20. Holaway BL, Kao G, Finn MC, Clancy MJ (1987) Mol Gen Gent 210:449–459Google Scholar
  21. Holmes D, Quigley M (1981) Anal Biochem 114:193–197Google Scholar
  22. Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168Google Scholar
  23. Kaback DB, Oeller PW, Steensma HY, Hirschman J, Ruezinsky D, Coleman KG, Pringle JR (1984) Genetics 108:67–90Google Scholar
  24. Kaback DB, Feldberg FR (1985) Mol Cell Biol 5:751–761Google Scholar
  25. Kao G, Mannix DG, Holaway BL, Finn MC, Bonny AE, Clancy MJ (1989) Mol Gen Genet 215:490–500Google Scholar
  26. Kassir Y, Granot D, Simchen G (1988) Cell:853–862Google Scholar
  27. Law DS, Segall J (1988) Mol Cell Biol 8:912–922Google Scholar
  28. Lindquist S, Craig EA (1988) Annu Review Genet 22:631–677Google Scholar
  29. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  30. Messing J (1983) Methods Enzymol 101:20–78Google Scholar
  31. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  32. Mortimer RK, Schild D, Contopoulou CR, Kans JA (1989) Yeast 5:321–403Google Scholar
  33. Osley MA, Hereford LM (1981) Cell 24:377–384Google Scholar
  34. Percival-Smith A, Segall J (1984) Mol Cell Biol 4:142–150Google Scholar
  35. Percival-Smith A, Segall J (1986) Mol Cell Biol 6:2443–2457Google Scholar
  36. Rigby PW, Dieckmann M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251Google Scholar
  37. Rose M, Botstein D (1983) Methods Enzymol 101:167–180Google Scholar
  38. Rothstein R (1983) Methods Enzymol 101:202–211Google Scholar
  39. Ruby S, Szostak JW, Murray AW (1983) Methods Enzymol 101:253–269Google Scholar
  40. Russell PW, Jensen R, Zoller MJ, Burke J, Errede B, Smith M, Herskowitz I (1986) Mol Cell Biol 6:4281–4294Google Scholar
  41. Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  42. Sentenac A, Hall B (1982) In: Strathern JN, Jones EW, Broach JR (eds) Molecular Biology of the yeast Saccharomyces. Metabolism and gene expression. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 561–606Google Scholar
  43. Sherman F, Fink GR, Hicks JB (1981) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  44. Sikorski RS, Hieter P (1989) Genetics 122:19–27Google Scholar
  45. Smith HE, Mitchell AP (1989) Mol Cell Biol 9:2142–2152Google Scholar
  46. Southern EM (1975) J Mol Biol 98:503–517Google Scholar
  47. Steensma HY, Crowley JC, Kaback DB (1987) Mol Cell Biol 7:410–419Google Scholar
  48. St. John TP, Davis RW (1979) Cell 16:443–452Google Scholar
  49. Tabor S, Richardson CC (1987) Proc Natl Acad Sci USA 84:4767–4771Google Scholar
  50. Thomas PS (1983) Methods Enzymol 100:255–266Google Scholar
  51. Wang H-T, Frackman S, Kowalisyn J, Esposito RE, Elder R (1987) Mol Cell Biol 7:1425–1435Google Scholar
  52. Williamson DH, Fennel DJ (1975) Methods Cell Biol 12:335–351Google Scholar
  53. Yamashita I, Fukui S (1985) Mol Cell Biol 5:3069–3073Google Scholar
  54. Yarger JG, Armilei G, Gorman MC (1986) Mol Cell Biol 6:1095–1101Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • L. A. Kallal
    • 1
  • M. Bhattacharyya
    • 3
  • S. N. Grove
    • 2
  • R. F. Iannacone
    • 3
  • T. A. Pugh
    • 3
  • D. A. Primerano
    • 4
  • M. J. Clancy
    • 1
  1. 1.Department of Biological SciencesUniversity of New OrleansNew OrleansUSA
  2. 2.Department of BiologyGoshen CollegeGoshenUSA
  3. 3.Department of Biological SciencesUniversity of Notre DameNotre DameUSA
  4. 4.Department of MicrobiologyMarshall University School of MedicineHuntingtonUSA

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