Current Genetics

, Volume 22, Issue 4, pp 337–339

High-level resistance to cycloheximide resulting from an interaction of the mutated pdr3 and cyh genes in yeast

  • Branislav Ruttkay-Nedecký
  • Margita Obernauerová
  • Július Šubík
Short Communications


In addition to pdr3-1, the S. cerevisiae nuclear pleiotropic drug resistance mutant 2D was found to contain another recessive nuclear mutation, cyh, conferring specific resistance to cycloheximide only. The cycloheximide resistance level due to either the pdr3-1 or the cyh mutation alone was low and was not altered by the ogd1 mutation which increased the physiological acidification of the culture. When pdr3-1 and cyh mutations occurred simultaneously in the haploid yeast strain their interaction was synergistic and resulted in high-level resistance to cycloheximide.

Key words

Gene interaction Multiple drug resistance Cycloheximide resistance Acidification 


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Branislav Ruttkay-Nedecký
    • 1
  • Margita Obernauerová
    • 1
  • Július Šubík
    • 1
  1. 1.Department of Microbiology and VirologyComenius UniversityBratislavaCzechoslovakia

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