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Current Genetics

, Volume 22, Issue 4, pp 313–316 | Cite as

Transformation of Gibberella fujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration

  • Bettina Brückner
  • Shiela E. Unkles
  • Klaus Weltring
  • James R. Kinghorn
Original Articles

Summary

A stable and reproducible transformation selection system for Gibberella fujikuroi protoplasts based on the Aspergillus nidulans arg B gene, encoding ornithine transcarbamylase, has been developed. Inclusion into the vector of the A. nidulans DNA fragment (AMA1), which permits plasmid autonomous replication in A. nidulans, A. niger and A. oryzae, appeared to permit autonomous replication of G. fujikuroi although the transformation frequency was increased by only two-fold. Transformation was also achieved using the bacterial hygromycin B resistance gene under the control of G. pulicaris and A. nidulans promoters.

Key words

Filamentous fungi Genetic transformation Arginine auxotrophy Hygromycin resistance Gibberellins 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Bettina Brückner
    • 1
  • Shiela E. Unkles
    • 2
  • Klaus Weltring
    • 3
  • James R. Kinghorn
    • 2
  1. 1.Institute of MicrobiologyFriedrich Schiller University of JenaJenaFederal Republic of Germany
  2. 2.Plant Molecular Genetics UnitUniversity of St. AndrewsSt. AndrewsScotland, UK
  3. 3.Institute of BotanyWestfälische Wilhelmsuniversität MünsterMünsterFederal Republic of Germany

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