Current Genetics

, Volume 22, Issue 4, pp 277–282

The REV2 gene of Saccharomyces cerevisiae: cloning and DNA sequence

  • F. Ahne
  • M. Baur
  • F. Eckardt-Schupp
Original Articles

Summary

The REV2 gene of Saccharomyces cerevisiae was cloned and sequenced; it contains an open reading frame of 1985 bp with a coding potential of 662 amino acids. Interruption of the chromosomal REV2 gene by integrating the URA3 gene coupled with partial deletion of the 3′ terminal region produced viable haploid rev2Δ mutants. This indicates that the REV2 gene is non-essential for growth. The rev2Δ mutant is slightly more UV-sensitive than strains carrying various rev2 alleles (rev2-1, rev2x, rad5-1, rad5-8). The putative Rev2 protein is probably a globular protein containing a highly conserved nucleotide-binding site and two zinc-finger domains.

Key words

Yeast DNA-repair Mutation-deficient mutant Nucleotide-binding consensus 

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References

  1. Ahne F, Wendel S, Eckardt-Schupp F (1990) Radiat Envir Biophys 29:293–301Google Scholar
  2. Berg JM (1986) Science 232:485–487Google Scholar
  3. Biggin MD, Gibson TJ, Son PH (1983) Proc Natl Acad Sci USA 80:3963–3965Google Scholar
  4. Birnboim H, Doly J (1979) Nucleic Acids Res 7:1513–1523Google Scholar
  5. Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW (1977) Gene 2:95–113Google Scholar
  6. Boyer HW, Roulland-Dussoix D (1969) J Mol Biol 41:459–472Google Scholar
  7. Budd M, Mortimer R (1982) Mutat Res 103: 19–24Google Scholar
  8. Carlson and Botstein (1982) Cell 28:145–154Google Scholar
  9. Chanet B, Magana-Schwencke N, Fabre F (1988) Gene 74:543–547Google Scholar
  10. Chanet B, Magana-Schwencke N, Fabre F (1988) Gene 74:543–547Google Scholar
  11. Cryer D, Eccleshall R, Eccleshall R, Marmur J (1975) In: Prescott DM (ed) Yeast cells, Methods Cell Biol Vol 12. Academic Press, New York, pp 139–144Google Scholar
  12. Emery HS, Schild D, Kellog DE, Mortimer RK (1991) Gene 104:103–106Google Scholar
  13. Finch PW, Emmerson PT (1984) Nucleic Acids Res 12:5789–5799Google Scholar
  14. Finch PW, Storey A, Chapman KE, Brown K, Hickson ID, Emmerson PT (1986) Nucleic Acids Res 14:8573–8582Google Scholar
  15. Fitzgerald M, Shenk T (1981) Cell 24:251–260Google Scholar
  16. Foury F, Lahaye A (1987) EMBO J 6:1441–1449Google Scholar
  17. friedberg EC (1988) Microbiol Rev 52:70–102Google Scholar
  18. Friedberg EC, Siede W, Cooper AJ (1991) In: Broach JR, Pringle JR, Jones EW (eds) The molecular and cell biology of the yeast Saccharomyces cerevisiae, genome dynamics, protein synthesis, and energetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 147–192Google Scholar
  19. Game J, Mortimer R (1974) Mutat Res 24:281–292Google Scholar
  20. Garmier J, Ostguthorpe DJ, Robson B (1978) J Mol Biol 120:97–120Google Scholar
  21. Geigl E-M, Eckardt-Schupp F (1991) Mol Microbiol 4(5):801–810Google Scholar
  22. Geigl E-M, Eckardt-Schupp F (1991) Curr Genet 20:33–37Google Scholar
  23. Haynes R, Kunz B (1981) In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces cerevisiae, life cycle and inheritance. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 371–414Google Scholar
  24. Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933Google Scholar
  25. Hussain I, Van Houten B, Thomas DC, Sancar A (1986) J Biol Chem 261:4895–4901Google Scholar
  26. Jones JS, Weber S, Prakash L (1988) Nucleic Acids Res 16:7119–7131Google Scholar
  27. Klug A, Rhodes D (1987) Trends Biochem Sci 12:464–469Google Scholar
  28. Kozak M (1983) Microbiol Rev 47:1–47Google Scholar
  29. Kuo C, Campbell J (1983) Mol Cell Biol 3:1730–1737Google Scholar
  30. Lawrence C (1982) Adv Genet 21:173–254Google Scholar
  31. Lawrence C, Christensen R (1978) Genetics 90:213–226Google Scholar
  32. Lawrence C, Kraus R, Christensen B (1985) Mutat Res 150:211–216Google Scholar
  33. Lederberg E, Cohen S (1974) J Bacteriol 119:1072–1074Google Scholar
  34. Lemontt JF (1971) Genetics 68:21–33Google Scholar
  35. Lemontt JF (1980) In: Generoso W, Shelby M, de Serres F (eds) DNA repair and mutagenesis in eukaryotes. Plenum Press, New York pp 85–120Google Scholar
  36. Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  37. Miller J, Mc Lachlan AD, Klug A (1985) EMBO J 4:1609–1614Google Scholar
  38. Mortimer R, Schild D (1985) Microbiol Rev 49:181–212Google Scholar
  39. Petes TD, Malone RE, Lorraine SS (1991) In: Broach JR, Pringle JR, Jones EW (eds) The molecular and cell biology of the yeast Sacccharomyces cerevisiae, genome dynamics, protein synthesis, and energetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 407–521Google Scholar
  40. Reynolds P, Higgins DR, Prakash L, Prakash S (1985) Nucleic Acids Res 13:2357–2372Google Scholar
  41. Rothstein RJ (1983) Methods Enzymol 101:202–211Google Scholar
  42. Saeki T, Machida I, Nakai S (1981) Mutat Res 73:251–265Google Scholar
  43. Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  44. Siede W, Eckardt F (1984) Mutat Res 129:3–11Google Scholar
  45. Siede W, Eckardt F (1986a) Mol Gen Genet 202:68–74Google Scholar
  46. Siede W, Eckardt F (1986b) Curr Genet 10:871–878Google Scholar
  47. Siede W, Eckardt-Schupp F (1986a) Curr Genet 11:205–210Google Scholar
  48. Siede W, Eckardt-Schupp F (1986b) Mutagenesis 1:471–474Google Scholar
  49. Siede W, Eckardt F, Brendel M (1983a) Mol Gen Genet 190:406–412Google Scholar
  50. Siede W, Eckardt F, Brendel M (1983b) Mol Gen Genet 190:413–416Google Scholar
  51. Sigurdson DC, Gaarder ME, Livingston DE (1981) Mol Gen Genet 183:59–65Google Scholar
  52. Sung P, Prakash L, Weber S, Prakash S (1987a) Proc Natl Acad Sci USA 84:6045–6049Google Scholar
  53. Sung P, Prakash L, Matson SW Prakash S (1987b) Proc Natl Acad Sci USA 84:8951–8955Google Scholar
  54. Verdier J (1990) Yeast 6:27–297Google Scholar
  55. Walker GC, Marsh L, Dodson LA (1985) Annu Rev Genet 19:104–126Google Scholar
  56. Walker JE, Saraste M, Runswick MJ, Gay NJ (1982) EMBO J 1:945–951Google Scholar
  57. Zaret K, Sherman F (1982) Cell 28:563–573Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • F. Ahne
    • 1
  • M. Baur
    • 1
  • F. Eckardt-Schupp
    • 1
  1. 1.GSF-Forschungszentrum für Umwelt und GesundheitInstitut für StrahlenbiologieNeuherbergFederal Republic of Germany

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