, Volume 88, Issue 4, pp 597–604 | Cite as

Reduced photoinhibition with stem curling in the resurrection plant Selaginella lepidophylla

  • Jefferson G. Lebkuecher
  • William G. Eickmeier
Original Papers


Selaginella lepidophylla, the resurrection plant, curls dramatically during desiccation and the hypothesis that curling may help limit bright light-induced damage during desiccation and rehydration was tested under laboratory conditions. Restraint of curling during desiccation at 25° C and a constant irradiance of 2000 μmol m−2 s]t-1 significantly decreased PSII and whole-chain electron transport and the Fv/Fm fluorescence yield ratio following rehydration relative to unrestrained plants. Normal curling during desiccation at 37.5°C and 200 μmol m−2 s−1 irradiance did not fully protect against photoinhibition or chlorophyll photooxidation indicating that some light-induced damage occurred early in the desiccation process before substantial curling. Photosystem I electron transport was less inhibited by high-temperature, high-irradiance desiccation than either PSII or whole-chain electron transport and PSI was not significantly affected by restraint of curling during desiccation at 25°C and high irradiance. Previous curling also helped prevent photoinhibition of PSII electron transport and loss of whole-plant photosynthetic capacity as the plants uncurled during rehydration at high light. These results demonstrate that high-temperature desiccation exacerbated photoinhibition, PSI was less photoinhibited than PSII or whole-chain electron transport, and stem curling ameliorated bright light-induced damage helping to make rapid recovery of photosynthetic competence possible when the plants are next wetted.

Key words

Desiccation Photoinhibition Photooxidation Selaginella lepidophylla 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24: 11–15Google Scholar
  2. Björkman O (1981) Responses to different quantum flux densities. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Physiological plant ecology I. (Encyclopedia of Plant Physiology, NS, vol 12A) Springer-Verlag, Berlin Heidelberg, pp 325–378Google Scholar
  3. Björkman O, Badger MR, Armond PA (1980) Response and adaptation of photosynthesis to high temperatures In: Turner NC, Kramer PJ (eds) Adaptation of plant to water and high temperature stress. Wiley, NY pp 233–249Google Scholar
  4. Björkman O, Demmig B (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77K among vascular plants of diverse origin. Planta 170:489–504Google Scholar
  5. Björkman O, Powles SB (1984) Inhibition of photosynthetic reactions under water stress: interaction with light level. Planta 161:490–504Google Scholar
  6. Chetti MB, Nobel PS (1987) High-temperature sensitivity and its acclimation for photosynthetic electron transport reactions of desert succulents. Plant Physiol 84:1063–1067Google Scholar
  7. Cleland RE, Melis A (1987) Probing the events of photoinhibition: photochemical reaction center inactivation in system II of chloroplasts. Photosyn Res 9:79–88Google Scholar
  8. Critchley C (1981) Studies on the mechanism of photoinhibition in higher plants. I. Effects of high light on chloroplast activities in cucumber adapted to low light. Plant Physiol 67:1161–1165Google Scholar
  9. Critchley C (1988) The molecular mechanism of photoinhibition-facts and fiction. Aust J Plant Physiol 15:27–41Google Scholar
  10. Demmig-Adams B (1990) Carotenoids and photoprotection in plants: a role for the xanthophyll zeaxanthin. Biocim Biophys Acta 1020:1–24Google Scholar
  11. Demmig-Adams B, Maguas C, Adams III WW, Meyer A, Kiliam E, Lange OL (1990) Effect of high light on the efficiency of photochemical energy conversion in a variety of lichen species with green and blue-green phycobionts. Planta 180:400–409Google Scholar
  12. Demmig-Adams B, Winter K Kruger A, Czygan F-C (1988) Zeaxanthin and the heat dissipation of excess light energy in Nerium oleander exposed to a combination of high light and water stress. Plant Physiol 87:17–24Google Scholar
  13. Eickmeier WG (1979) Photosynthetic recovery in the resurrection plant Selaginella lepidophylla after wetting. Oecologia 39:93–106Google Scholar
  14. Eickmeier WG (1980) Photosynthetic recovery of resurrection spikemosses from different hydration regimes. Oecologia 46:380–385Google Scholar
  15. Eickmeier WG (1983) Photosynthetic recovery of the resurrection plant Selaginella lepidophylla (Hook. and Grev.) Spring: effects of prior desiccation rate and mechanisms of desiccation damage. Oecologia 58:115–120Google Scholar
  16. Eickmeier WG (1986) The correlation between high-temperature and desiccation tolerances in a poikilohydric desert plant. Can J Bot 54:611–61Google Scholar
  17. Ferrar PJ, Osmond CB (1986) Nitrogen supply as a factor influencing photoinhibition and photosynthetic acclimation after transfer of shade-grown Solanum dulcamara to bright light. Planta 168:563–570Google Scholar
  18. Forseth I, Ehleringer JR (1980) Solar tracking response to drought in a desert annual oecologia 44:159–163Google Scholar
  19. Foyer C, Furbank R, Harbinson J, Horton P (1990) The mechanisms contributing to photosynthetic control of electron transport by carbon assimilation in leaves. Photosyn Res 25:83–100Google Scholar
  20. Gauhl E (1976) Photosynthetic response to varying light intensity in ecotypes of Solanum dulcamara L. from shaded and exposed environments. Oecologia 22:275–28Google Scholar
  21. Kitajima M, Butler WL (1975) Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochim Biophys Acta 376:105–115Google Scholar
  22. Krause GH (1988) Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms. Physiol Plant 74:566–574Google Scholar
  23. Ludlow MM, Björkman O (1983) Paraheliotrophic leaf movement as a protective mechanism against drought-induced damage to primary photosynthetic reactions. Carnegie Inst Washington Yearbk 82:89–91Google Scholar
  24. Ludlow MM, Björkman O (1984) Paraheliotrophic leaf movement in Siratro as a protective mechanism against drought-induced damage to primary photosynthetic reactions: damage by excessive light and heat. Planta 161:505–518Google Scholar
  25. Ögren E, Öquist G (1985) Effect of drought on photosynthesis, chlorophyll fluorescence and photoinhibition suseptibility in intact willow leaves. Planta 180:400–409Google Scholar
  26. Powles SB,(1984) Photoinhibition of photosynthesis induced by visible light. Ann Rev Plant Physiol 35:15–44Google Scholar
  27. Powles SB, Björkman O (1981) Leaf movement in the shade species Oxalis oregana. II. Role in protection against injury by intense light. Carnegie Inst Washington Yearbk 80:63–66Google Scholar
  28. Powles SB, Critchley C (1980) Effect of light intensity during growth on photoinhibition of attached bean leaflets. Plant Physiol 65:1181–1187Google Scholar
  29. Powles SB, Osmond CB, Thorn SW (1979) Photoinhibition of attached leaves of C3 plants illuminated in the absence of both carbon dioxide and of photorespiration. Plant Physiol 64:982–988Google Scholar
  30. Prichard JM, Forseth IN (1988) Rapid leaf movement, microclimate, and water relations of two temperate legumes in three contrasting habitats. Amer J Bot 75(8):1201–1211Google Scholar
  31. Satoh K, Fork DC (1982) Photoinhibition of reaction centers of photosystems I and II in intact Bryopsis chloroplasts illuminated under anaerobic conditions. Photobiochem Photobiophys 4:153–162Google Scholar
  32. Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical fluorescence quenching with a new type of modulation fluorometer. Photosyn Res 10:51–62Google Scholar
  33. Sokal RR, Rohlf FJ (1981) Biometry (Second Ed) WH Freeman and Company, NYGoogle Scholar
  34. Zar JE (1984) Biostatistical analysis (Second Ed) Prentice-Hall Englewood Cliffs, NJGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Jefferson G. Lebkuecher
    • 1
  • William G. Eickmeier
    • 1
  1. 1.Department of BiologyVanderbilt UniversityNashvilleUSA

Personalised recommendations