Anatomy and Embryology

, Volume 155, Issue 3, pp 355–362 | Cite as

Spinal ganglion cells innervating the stomach of the rat as demonstrated by somatopetal transport of Horseradish peroxidase (HRP)

  • Winfried Neuhuber
  • Bruno Niederle


Spinal ganglion cells innervating the stomach of the rat were demonstrated using the somatopetal horseradish peroxidase (HRP) transport technique. After injection of the tracer into the anterior wall of the stomach, labelled neurons were observed bilaterally within spinal ganglia T4-L1. They were most numerous in ganglia T8-T10. No clear evidence of somatotopic organization of the labelled cells was found.

Most of the cells measured 35–40 μm in diameter. There was a striking paucity of cells smaller than 30 μm. The predominance of medium-sized spinal ganglion cells may indicate that the stomach is innervated mainly by small myelinated rather than unmyelinated splanchnic afferent fibers.

Key words

Visceral afferents Spinal ganglion cells Stomach HRP Somatopetal transport 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alvarez, W.C.: Abdominal pain. Amer. J. Surg. 14, 385–394 (1931)Google Scholar
  2. Bain, W.A., Irving, J.T., McSwiney, B.A.: The afferent fibres from the abdomen in the splanchnic nerves. J. Physiol. (London) 84, 323–333 (1935)Google Scholar
  3. Broadwell, R.D., Brightman, M.W.: Entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral and cerebral blood. J. comp. Neur. 166, 257–284 (1976)Google Scholar
  4. Coggeshall, R.E., Hancock, M.B., Applebaum, M.L.: Categories of axons in mammalian rami communicantes. J. comp. Neur. 167, 105–124 (1976)Google Scholar
  5. Elfvin, L.-G., Dalsgaard, C.J.: Retrograde axonal transport of horseradish peroxidase in afferent fibers of the inferior mesenteric ganglion of the guinea pig. Identification of the cells of origin in dorsal root ganglia. Brain Res. 126, 149–153 (1977)Google Scholar
  6. Ellison, J.P., Clark, G.M.: Retrograde axonal transport of horseradish peroxidase in peripheral autonomic nerves. J. comp. Neur. 161, 103–114 (1975)Google Scholar
  7. Gabella, G.: Structure of the autonomic nervous system. London: Chapman and Hall 1976Google Scholar
  8. Graham, R.C. and Karnovsky, M.J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. histochem. cytochem. 14, 291–302 (1966)Google Scholar
  9. Irving, J.T., McSwiney, B.A., Suffolk, S.F.: Afferent fibres from the stomach and small intestine. J. Physiol. (London) 89, 407–420 (1937)Google Scholar
  10. Kristensson, K., Olsson, Y., Sjöstrand, J.: Axonal uptake and retrograde transport of exogenous proteins in the hypoglossal nerve. Brain Res. 32, 399–406 (1971)Google Scholar
  11. Leek, B.F.: Abdominal visceral receptors. In: Handbook of sensory physiology (ed. Neil, E.), Vol. 3. Berlin: Springer 1972Google Scholar
  12. Leek, B.F.: Abdominal and pelvic visceral receptors. Br. med. Bull. 33, 163–168 (1977)Google Scholar
  13. Li, C.L., Mathews, G., Bak, A.F.: Action potential of somatic and autonomic nerves. Exp. neurol. 56, 527–537 (1977)Google Scholar
  14. Lieberman, A.R.: Sensory ganglia. In: The peripheral nerve (ed. D.N. Landon), London: Chapman and Hall 1976Google Scholar
  15. Neuhuber, W., Niederle, B., Zenker, W.: Somatopetal transport of horseradish peroxidase (HRP) in the peripheral and central branches of dorsal root ganglion cells. Cell Tiss. Res. 183, 395–402 (1977)Google Scholar
  16. Newman, P.P.: Visceral afferent functions of the nervous system. London: Arnold 1974Google Scholar
  17. Paintal, A.S.: Vagal sensory receptors and their reflex effects. Physiol. Rev. 53, 159–227 (1973)Google Scholar
  18. Ranson, S.W. and Billingsley, P.R.: An experimental analysis of the sympathetic trunk and greater splanchnic nerve in the cat. J. comp. Neur. 29, 441–456 (1918)Google Scholar
  19. Texter, E.C.: Digestive tract pain. Amer. J. dig. Dis. 3, 877–900 (1958)Google Scholar
  20. Warrington, W.B., Griffith, F.: On the cells of the spinal ganglia and on the relationship of their histological structure to their axonal distribution. Brain 27, 297–326 (1904)Google Scholar
  21. Yamamoto, T., Satomi, H., Ise, H., Takahashi, K.: Evidence of the dual innervation of the cat stomach by the vagal dorsal motor and medial solitary nuclei as demonstrated by the horseradish peroxidase method. Brain Res. 122, 125–131 (1977)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Winfried Neuhuber
    • 1
    • 2
  • Bruno Niederle
    • 1
    • 2
  1. 1.II. Anatomisches Institut der Universität WienWienÖsterreich
  2. 2.Anatomisches Institut der Universität ZürichZürichSchweiz

Personalised recommendations