Current Genetics

, Volume 20, Issue 5, pp 373–378

PDC6, a weakly expressed pyruvate decarboxylase gene from yeast, is activated when fused spontaneously under the control of the PDC1 promoter

  • Stefan Hohmann
Original Articles

Summary

Three structural genes encode the pyruvate decarboxylase isoenzymes in the yeast Saccharomyces cerevisiae. PDC1 and PDC5 are active during glucose fermentation where PDC1 is expressed about six times more strongly than PDC5. Expression of PDC6 is weak and seems to be induced in ethanol medium. Consequently, pdc1Δ pdc5Δ double mutants do not ferment glucose and do not grow on glucose medium. Spontaneous mutants, derived from such a pdc1 pdc5 strain, were isolated which could again ferment glucose. They showed pyruvate decarboxylase activity due to a duplication of PDC6. The second copy of PDC6 was expressed under the control of the PDC1 promoter, which was still present in the pdc1 strain. However, the resulting PDC1-PDC6 fusion gene could only partially substitute for PDC1: to achieve normal growth and high pyruvate decarboxylase activity strains carrying PDC1-PDC6 required a functional PDC5 gene which is dispensable in a PDC1 wild-type background. Thus, expression of PDC5 depends on the state of the PDC1 locus: low in the PDC1 wild-type background and high in PDC1-PDC6 fusion strains and, as shown previously, in pdc1 mutants. The activation of PDC5 expression in PDC1-PDC6 strains may be due to particular properties of the PDC1-PDC6 fusion protein or simply to the weaker expression of PDC1-PDC6 in comparison to the wild-type PDC1 gene.

Key words

Yeast Pyruvate decarboxylase Gene expression Codon usage Gene fusion 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andersson SGE, Kurland CG (1990) Microbiol Rev 54:198–210Google Scholar
  2. Bennetzen JL, Hall BD (1982a) J Biol Chem 257:3018–3025Google Scholar
  3. Bennetzen JL, Hall BD (1982b) J Biol Chem 257:3026–3031Google Scholar
  4. Entian K-D (1986) Microbiol Sci 3:366–371Google Scholar
  5. Denis CL, Ferguson J, Young ET (1981) J Mol Biol 148:355–368Google Scholar
  6. Gallwitz D, Sures J (1980) Proc Natl Acad Sci USA 77:2546–2550Google Scholar
  7. Gancedo C, Serrano R (1989) In: Rose AH, Harrison JS (eds). The Yeasts vol 3, 2nd edn. Academic Press, New York, pp 205–259Google Scholar
  8. Hohmann S, Zimmermann FK (1986) Curr Genet 11:217–225Google Scholar
  9. Hohmann S, Cederberg H (1990) Eur J Biochem 188:615–621Google Scholar
  10. Russell DW, Smith M, Williamson VM, Young ET (1983) J Biol Chem 258:2674–2682Google Scholar
  11. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  12. Sanger F, Nicklen S, Coulen AR (1977) Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  13. Schaaff I, Green JBA, Gozalbo D, Hohmann S (1989) Curr Genet 15:75–81Google Scholar
  14. Schmitt HD, Zimmermann FK (1982) J Bacteriol 151:1146–1152Google Scholar
  15. Seeboth PG, Bohnsack K, Hollenberg CP (1990) J Bacteriol 172:678–685Google Scholar
  16. Sharp PM, Tuohy TMF, Mosurski KR (1986) Nucleic Acids Res 14:5125–5143Google Scholar
  17. Sherman F, Fink GR, Hicks JB (1986) Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  18. Yanish-Perron C, Vieria J, Messing J (1985) Gene 33:103–109Google Scholar
  19. Zamenhoff S (1957) Methods Enzymol 3:696–704Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Stefan Hohmann
    • 1
    • 2
  1. 1.Institut für MikrobiologieTechnische Hochschule DarmstadtDarmstadtFederal Republic of Germany
  2. 2.Labo voor Moleculaire Celbiologie, Instituut voor PlantkundeKatholieke Universiteit te LeuvenLeuven-Heverlee, FlandersBelgium

Personalised recommendations