European Journal of Clinical Pharmacology

, Volume 44, Issue 4, pp 315–318 | Cite as

Anti-inflammatory activity of hamamelis distillate applied topically to the skin

Influence of vehicle and dose
  • H. C. Korting
  • M. Schäfer-Korting
  • H. Hart
  • P. Laux
  • M. Schmid


The anti-inflammatory activity of hamamelis distillate has been evaluated with respect to drug concentration (0.64 mg/2.56 mg hamamelis ketone/100 g) and the effect of the vehicle (O/W emulsion with/without phosphatidylcholine (PC) in an experimental study. The effects were compared with those of chamomile cream, hydrocortisone 1% cream and 4 base preparations. Erythema was induced by UV irradiation and cellophane tape stripping of the horny layer in 24 healthy subjects per test. Skin blanching was quantified by visual scoring and chromametry. Drug effects were compared with one another and with an untreated control area, as well as with any action due to the vehicle.

UV-induced erythema at 24 h was suppressed by low dose hamamelis PC-cream and hydrocortisone cream. Hydrocortisone appeared superior to both hamamelis vehicles, hamamelis cream (without PC) and chamomile cream. The latter preparation was also less potent than hamamelis PC-cream. Erythema 4 to 8 h after the stripping of the horny layer was suppressed by hydrocortisone (P≤0.05). Inflammation was also less pronounced following low dose hamamelis PC-cream and chamomile cream. Hamamelis PC-cream, however, appeared less potent than hydrocortisone. In general, visual scoring was more discriminatory than chromametry.

The results have demonstrated an anti-inflammatory activity of hamamelis distillate in a PC-containing vehicle. A fourfold increase of drug concentration, however, did not produce an increase in activity.

Key words

Hamamelis Chamomile Hydrocortisone anti-inflammatory activity vehicle effects UV-erythema test cellophane tape stripping phosphatidyl choline 


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  1. 1.
    Bateman DN (1989) Clinical pharmacology of topical steroids. In: Greaves MW, Shuster S (eds) Pharmacology of the Skin II. Springer, Berlin Heidelberg New York, pp 239–249Google Scholar
  2. 2.
    Robertson DB, Maibach HI (1989) Topical glucocorticoids. In: Schleimer RP, Claman HN, Oronsky A (eds) Anti-inflammatory steroid action. Basic and clinical aspects. Academic Press, San Diego, pp 494–524Google Scholar
  3. 3.
    Schäfer-Korting M (1993) Topical glucocorticoids: what has been achieved; what is still to be done. In: Korting HC, Maibach HI (eds) Topical glucocorticoids with increased benefit-risk-ratio. Karger, Basle (in press)Google Scholar
  4. 4.
    Niedner R (1991) Grundlagen einer rationalen Therapie mit externen Glukokortikosteroiden. Hautarzt 42: 337–346Google Scholar
  5. 5.
    Aertgeerts P, Albring M, Klaschka F, Nasemann Th, Patzelt-Wenczler R, Rauhut K, Weigl B (1985) Vergleichende Prüfung von Kamillosan® Creme gegenüber steroidalen (0,25% Hydrocortison, 0,75% Fluocortinbutylester) und nichtsteroidalen (5% Bufexamac) Externa in der Erhaltungstherapie von Ekzemerkrankungen. Zeitschr Hautkrankh 60: 270–277Google Scholar
  6. 6.
    Peters H (1988) Suchdiät und Neurodermitis. In: Klaschka F, Maiwald L, Patzelt-Wenczler R (eds) Wirkungsweise und Anwendungsformen der Kamille. Grosse Verlag, Berlin, pp 45–48Google Scholar
  7. 7.
    Pfister R (1981) Zur Problematik der Behandlung und Nachbehandlung chronischer Dermatosen. Eine klinische Studie über Hametum Salbe. Fortschr Med 99: 1264–1268Google Scholar
  8. 8.
    Schweiker RS (1982) Skin protectant drug products for over the counter human use; establishment of a monograph; and the reopening of administrative record. Fed Reg (USA) 47: 39436–39451Google Scholar
  9. 9.
    Sorkin B (1980) Hametum Salbe, eine kortikoidfreie antiinflammatorische Salbe. Phys Med Rehab 21: 53–57Google Scholar
  10. 10.
    Della Loggia R (1985) Lokale antiphlogistische Wirkung der Kamillen-Flavone. Dtsch Apoth Ztg 125 [Suppl 1]: 9–11Google Scholar
  11. 11.
    Albring M, Albrecht H, Alcorn G, Lücker PW (1983) The measuring of the antiinflammatory effect of a compound on the skin of volunteers. Meth Find Exp Clin Pharmacol 5: 575–577Google Scholar
  12. 12.
    Nissen HP, Biltz H, Kreysel HW (1988) Profilometrie, eine Methode zur Beurteilung der therapeutischen Wirksamkeit von Kamillosan® Salbe. Zeitschr Hautkrankh 63: 184–190Google Scholar
  13. 13.
    Gesztes A, Mezei M (1988) Topical anesthesia of the skin by liposome-encapsulated tetracaine. Anesth Analg (New York) 67: 1079–1081Google Scholar
  14. 14.
    Weiner N, Williams N, Birch G, Ramachandran C, Shipman C, Jr., Flynn G (1989) Topical delivery of liposomally encapsulated interferon evaluated in a cutaneous herpes guinea pig model. Antimicrob Agents Chemother 33: 1217–1221Google Scholar
  15. 15.
    Yarosh DB, Tsimis J, Yee V (1990) Enhancement of DNA repair of UV damage in mouse and human skin by liposomes containing a DNA repair enzyme. J Soc Cosmet Chem 41: 85–92Google Scholar
  16. 16.
    Korting HC, Zienicke H, Schäfer-Korting M, Braun-Falco O (1990) Liposome encapsulation improves efficacy of betamethasone dipropionate in atopic eczema but not in psoriasis vulgaris. Eur J Clin Pharmacol 39: 349–351Google Scholar
  17. 17.
    Jacobs M, Martin GP, Marriott C (1988) Effects of phosphatidylcholine on the topical bioavailability of corticosteroids assessed by the human skin blanching assay. J Pharm Pharmacol 40: 829–833Google Scholar
  18. 18.
    Ljunggren B, Möller H (1973) Influence of corticosteroids on ultraviolet light erythema and pigmentation in man. Arch Derm Forsch 248: 1–12Google Scholar
  19. 19.
    Wells GC (1957) The effect of hydrocortisone on standardized skin-surface trauma. Br J Dermatol 69: 11–18Google Scholar
  20. 20.
    Brogden RN, Pinder RM, Sawyer PR, Speight TM, Avery GS (1975) Bufexamac: a review of its pharmacological properties and therapeutic efficacy in inflammatory dermatoses. Drugs 10: 351–356Google Scholar
  21. 21.
    Schäfer-Korting M, Korting HC (1992) Ekzeme, Ekzemtherapie heute. Dtsch Apoth Ztg 132: 59–69Google Scholar
  22. 22.
    Greaves MW, Shuster S (1989) Non-steroidal anti-inflammatory agents and the skin. In: Greaves MW, Shuster S (eds) Pharmacology of the Skin II. Springer, Berlin Heidelberg New York, pp 301–305Google Scholar
  23. 23.
    Comaish JS (1987) The effect of tar and ultraviolet on the skin. J Invest Dermatol 88: 61s-64sGoogle Scholar
  24. 24.
    Elmets CA (1992) Cutaneous photocarcinogenesis. In: Mukhtar H (ed) Pharmacology of the Skin. CRC Press, Boca Raton, pp 389–416Google Scholar
  25. 25.
    Morse PF, Horrobin DF, Manku MS, Stewart JCM, Allen R, Littlewood S, Wright S, Burton J, Gould DJ, Holt PJ, Jansen CT, Mattila L, Meigel W, Dettke Th, Wexler D, Guenther L, Bordoni A, Patrizi A (1989) Meta-analysis of placebo-controlled studies of the efficacy of Epogam in the treatment of atopic eczema. Relationship between plasma essential fatty acid changes and clinical response. Br J Dermatol 121: 75–90Google Scholar
  26. 26.
    Zesch A (1992) Clinical regulatory aspects of topical liposomal drugs with special consideration of safety aspects. In: Braun-Falco O, Korting HC, Maibach HI (eds) Liposome Dermatics. Springer, Berlin Heidelberg New York, pp 288–296Google Scholar
  27. 27.
    Wilhelm K-P, Surber C, Maibach HI (1989) Quantification of sodium lauryl sulfate irritant dermatitis in man: comparison of four techniques: skin color reflectance, transepidermal water loss, laser Doppler flow measurement and visual scores. Arch Dermatol Res 281: 293–295Google Scholar
  28. 28.
    Schäfer-Korting M, Korting HC, Braun-Falco O (1989) Liposome preparations: a step forward in topical drug therapy for skin disease? J Am Acad Dermatol 21: 1271–1275Google Scholar
  29. 29.
    Röding J (1992) Properties and characterization of preliposome systems. In: Braun-Falco O, Korting HC, Maibach HI (eds) Liposome Dermatics. Springer, Berlin Heidelberg New York, pp 110–117Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • H. C. Korting
    • 1
  • M. Schäfer-Korting
    • 2
  • H. Hart
    • 1
  • P. Laux
    • 3
  • M. Schmid
    • 1
  1. 1.Department of DermatologyLudwig-Maximilians-UniversitätMünchenGermany
  2. 2.Department of Pharmacology for ScientistsJohann Wolfgang Goethe-UniversitätFrankfurtGermany
  3. 3.W. Spitzner ArzneimittelEttlingenGermany

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