Histochemistry

, Volume 97, Issue 6, pp 493–497

Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with oil red O

  • J. L. Ramírez-Zacarías
  • F. Castro-Muñozledo
  • W. Kuri-Harcuch
Article

DOI: 10.1007/BF00316069

Cite this article as:
Ramírez-Zacarías, J.L., Castro-Muñozledo, F. & Kuri-Harcuch, W. Histochemistry (1992) 97: 493. doi:10.1007/BF00316069

Summary

Cultured 3T3-F442A cells differentiate into adipocytes and accumulate lipid droplets in the cytoplasm. When fat cells are stained with Oil red O, the degree of staining seems to be proportional to the extent of cell differentiation. We report here a fast and simple method to quantitate the extent of adipose conversion by staining the accumulated lipid with Oil red O and determining the amount of extracted dye at 510 nm. The results show that Oil red O specifically stains triglycerides and cholesteryl oleate but no other lipids. This technique is a valuable tool for processing large numbers of cell cultures or samples in which adipose differentiation and/or accumulated triglycerides is to be quantitated.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • J. L. Ramírez-Zacarías
    • 1
  • F. Castro-Muñozledo
    • 1
  • W. Kuri-Harcuch
    • 1
  1. 1.Department of Cell BiologyCentro de Investigación y de Estudios Avanzados del IPNMéxico CityMéxico
  2. 2.Department of Cell BiologyEscuela de Medicina de la Universidad Autónoma de San Luis PotosíSan Luis PotosiMexico
  3. 3.Biosurface Technology, Inc.CambridgeUSA

Personalised recommendations