Anatomy and Embryology

, Volume 177, Issue 4, pp 317–324 | Cite as

Effect of the notochord on the differentiation of a floor plate area in the neural tube of the chick embryo

  • H. W. M. van Straaten
  • J. W. M. Hekking
  • E. J. L. M. Wiertz-Hoessels
  • F. Thors
  • J. Drukker


The role of a notochord fragment on the origin of an additional floor plate area in the neural tube is investigated by quantitative morphological methods. In 1.5 to 2 day chick embryos a notochordal fragment was implanted in close apposition to the lateral wall of the neural groove in the region between prospective wing and leg bud. At 4 days, adjacent to the implant a distinct area of the neural wall was present, which resembled the natural floor plate with respect to its thickness, the abluminal location of elongated nuclei and the absence of neuroblasts. The mitotic density of this area was reduced. This “additional floor plate” was distinct when the experiment was performed at 1.5 days but was hardly recognizable when it was carried out at 2 days.

From these results it is concluded that a) the notochord induces floor plate like structures and diminishes proliferation, and b) that the period of floor plate induction by the notochord is very restricted.

Key words

Chick embryo Notochord Neural tube Floor plate Proliferation 


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  1. Bergquist H (1959) Experiments on the overgrowth phenomenon in the brain of chick embryos. Anat Anz 106:429–441Google Scholar
  2. Burda DJ (1968) Studies on the experimental induction of overgrowth in chick embryos. Anat Rec 161:419–425Google Scholar
  3. Corliss CE, Robertson GG (1963). The pattern of mitotic density in the early chick neural epithelium. J Exp Zool 153:125–140Google Scholar
  4. Fujita S (1962) Kinetics of cell proliferation. Exp Cell Res 28:52–60Google Scholar
  5. Hamburger V (1948) Kinetics of cell proliferation. Exp Cell Res 28:52–60Google Scholar
  6. Hamburger V (1948) The mitotic pattern in the spinal cord of the chick embryo in relation to histogenetic processes. J Comp Neurol 88:221–284Google Scholar
  7. Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92Google Scholar
  8. Holtfreter J, Hamburger V (1955) Amphibians. In: Willier BH, Weiss PA, Hamburger V (eds) Analysis of development. Saunders, Philadelphia, pp 230–296Google Scholar
  9. Jacob HJ, Christ B, Jacob M, Ahlström P (1976) Differenzierung des Neuralrohres. Experimentelle Untersuchungen an Hühnerund Wachtelembryonen. Acta Anat 94:204–220Google Scholar
  10. Jacobson AG (1978) Some forces that shape the nervous system. Zoon 6:13–21Google Scholar
  11. Jacobson AG, Oster GF, Odell GM, Cheng LY (1986) Neurulation and the cortical tractor model for epithelial folding. J Embryol Exp Morphol 96:19–49Google Scholar
  12. Jacobson C-O (1962) Cell migration in the neural plate and the process of neurulation in the axolotl larva. Zool Bidrag Uppsala 35:433–449Google Scholar
  13. Jacobson M (1979) Developmental Neurobiology (2nd edn) Plenum Press, New YorkGoogle Scholar
  14. Källén B (1965) Early Morphogenesis and pattern formation in the central nervous system. In: de Haan R, Ursprung H (eds) Organogenesis. Holt, Rhinehart and Winston, New York, pp 107–128Google Scholar
  15. Karfunkel P (1974) The mechanism of neural tube formation. Int Rev Cytol 38:245–271Google Scholar
  16. Karnovski MJ, Roots L (1964) A “direct colouring” thiocholine method for cholinesterase. J Histochem Cytochem 12:219–221Google Scholar
  17. Limborgh J van (1956) The influence of adjacent mesodermal structures upon the shape of the neural tube and plate in bird embryos. Acta Morphol Neerl Scand 1:155–166Google Scholar
  18. Martin AH (1977) Neural tube — somite interaction, effect of neural tube reversal upon neuron development. Acta Embryol Exp 3:305–313Google Scholar
  19. Miki A, Atoji Y, Mizoguti H (1981) The relationship between the proliferating ability and acetylcholinesterase activity of the neural tube cells in early chick embryos. Acta Histochem Cytochem 14:461–475Google Scholar
  20. Mitolo V (1967) Quantitative data on the mitotic activity in the brachial and thoracic segments of the chick embryo neural tube from the 4th to the 8th day. Acta Embryol Morphol Exp 10:62–74Google Scholar
  21. Sauer FC (1935) Mitosis in the neural tube. J Comp Neurol 62:377–405Google Scholar
  22. Schoenwolf GC, Franks MV (1984) Quantitative analysis of changes in cell shapes during bending of the avian neural plate. Dev Biol 105:257–272Google Scholar
  23. Schoenwolf GC (1985) Shaping and bending of the avian neuroepithelium: morphometric analyses. Dev Biol 109:127–139Google Scholar
  24. Steding G (1962) Experimente zur Morphogenese des Rückenmarkes. Acta Anat 49:199–231Google Scholar
  25. Straaten HWM van, Thors F, Hoessels EL, Hekking JWM, Drukker J (1985a) Effect of a notochordal implant on the early morphogenesis of the neural tube and neuroblasts. Histometrical and histological results. Dev Biol 110:247–254Google Scholar
  26. Straaten HWM van, Hekking JWM, Thors F, Wiertz ELJM, Drukker J (1985b) Induction of an additional floor plate in the neural tube. Acta Morphol Neerl Scand 23:91–97Google Scholar
  27. Straaten HWM van, Hekking JWM, Drukker J (1986) The demonstration of acetylcholinesterase in plastic sections. Its application as a marker of early neuronal development. Acta Histochem Suppl 32:185–190Google Scholar
  28. Straaten HWM, van Drukker J (1987a) Influence of the notochord on the morphogenesis of the neural tube. In: Wolff JR (ed) Mesenchymal-epithelial interactions. Academic Press, pp 153–163Google Scholar
  29. Straaten HWM van, Hekking J, Drukker J (1987b) A toluidin blue staining procedure selective for nuclei, mitotic figures and axial processes in GMA embedded embryonic neural tissue. Stain Technol (in press)Google Scholar
  30. Thors F, Kort EMJ de, Nieuwenhuys R (1982) On the development of the spinal cord of the clawed frog, Xenopus laevis. I. Morphogenesis and histogenesis. Anat Embryol 164:427–441Google Scholar
  31. Watterson RL, Goodheart C, Lindberg G (1955) The influence of adjacent structures upon the shape of the neural tube and neural plate of chick embryos. Anat Rec 122:539–559Google Scholar
  32. Watterson RL (1965) Structure and mitotic behavior of the early neural tube. In: Haan RL de, Ursprung H (eds) Organogenesis. Holt, Rhinehart and Winston, New York Chicago, pp 129–159Google Scholar
  33. Wiertz-Hoessels ELMJ, Hara K, Hekking JWM, van Straaten HWM, Thors F, Drukker J (1987) Differentiation of gut entoderm in dependence of the notochord. Anat Embryol 176:337–343Google Scholar
  34. Youn BW, Malacinsky GM (1981) Axial structure development in ultraviolet-irradiated (notochord-defective) amphibian embryos. Dev Biol 83:339–352Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • H. W. M. van Straaten
    • 1
  • J. W. M. Hekking
    • 1
  • E. J. L. M. Wiertz-Hoessels
    • 1
  • F. Thors
    • 1
  • J. Drukker
    • 1
  1. 1.Department of Anatomy and EmbryologyUniversity of LimburgMaastrichtThe Netherlands

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