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Current Genetics

, Volume 28, Issue 2, pp 138–149 | Cite as

As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombe

  • Marc Lollier
  • Laurence Jaquet
  • Triana Nedeva
  • François Lacroute
  • Serge Potier
  • Jean-Luc Souciet
Original Paper

Abstract

The organisation of the URA1 gene of Schizosaccharomyces pombe was determined from the entire cDNA cloned by the transformation of an ATCase-deficient strain of Saccharomyces cerevisiae. The URA1 gene encodes the bifunctional protein GLNase/CPSase-ATCase which catalyses the first two steps of the pyrimidine biosynthesis pathway. The complete nucleotide sequence of the URA1 cDNA was elucidated and the deduced amino-acid sequence was used to define four domains in the protein; three functional domains, corresponding to GLNase (glutamine amidotransferase), CPSase (carbamoylphosphate synthetase) and ATCase (aspartate transcarbamoylase) activities, and one cryptic DHOase (dihydroorotase) domain. Genetic investigations confirmed that both GLNase/CPSase and ATCase activities are carried out by the same polypeptide. They are also both feedback-inhibited by UTP (uridine triphosphate). Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product.

Key words

Schizosaccharomyces pombe Pyrimidine pathway Feedback inhibition DHOase-like Multifunctional protein 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Marc Lollier
    • 1
  • Laurence Jaquet
    • 1
  • Triana Nedeva
    • 2
  • François Lacroute
    • 3
  • Serge Potier
    • 1
  • Jean-Luc Souciet
    • 1
  1. 1.Laboratoire de Microbiologie et de GénétiqueURA n-1481 Université Louis-Pasteur/CNRSStrasbourg CedexFrance
  2. 2.Faculty of BiologyUniversity St Kliment OhridskiSofiaBulgaria
  3. 3.Centre de Génétique Moléculaire du CNRSGif-sur-Yvette CedexFrance

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