Anatomy and Embryology

, Volume 155, Issue 1, pp 87–94 | Cite as

The cerebellar projection from locus coeruleus as studied with retrograde transport of horseradish peroxidase in the cat

  • R. Somana
  • F. Walberg


The cerebellar afferent projection from locus coeruleus has been studied in the cat by means of retrograde axonal transport of horseradish peroxidase. Labelled cells are present bilaterally in locus coeruleus only following injections in the cerebellar vermis (especially its anterior and posterior parts), the ventral paraflocculus and the flocculus. The labelled cells are restricted to the caudal half of the nucleus.

A few labelled cells are also present in locus coeruleus following injections in the fastigial nucleus, and in nucleus interpositus anterior. The findings are discussed in relation to other studies on the efferent and afferent connections of the locus coeruleus.

Key words

Locus coeruleus Cerebellar afferent projections Peroxidase 


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  1. Andén, E., Dahlström, A., Fuxe, K., Larsson, K., Olson, L., Ungerstedt, U.: Ascending monoamine neurons to the telencephalon and diencephalon. Acta physiol. scand. 67, 313–326 (1966)Google Scholar
  2. Arikuni, T., Ban Jr., T.: Subcortical afferents to the prefrontal cortex in rabbits. Exp. Brain Res. 32, 69–75 (1978)Google Scholar
  3. Bloom, F.E., Hoffer, B.J., Siggins, G.R.: Studies on norepinephrine-containing afferents to Purkinje cells of rat cerebellum. I. Localization of the fibers and their synapses. Brain Research 25, 501–521 (1971)Google Scholar
  4. Bowden, D.M., German, D.C., Poynter, W.D.: An autoradiographic, semistereotaxic mapping of major projections from locus coeruleus and adjacent nuclei in Macaca Mulatta. Brain Research 145, 257–276 (1978)Google Scholar
  5. Brown, J.O.: The nuclear pattern of the non-tectal portions of the midbrain and isthmus in the dog and cat. J. Comp. Neurol. 78, 365–405 (1943)Google Scholar
  6. Cedarbaum, J.M., Aghajanian, G.K.: Afferent projections to the rat locus coeruleus as determined by a retrograde tracing technique. J. Comp. Neurol. 178, 1–16 (1978)Google Scholar
  7. Chu, N.-S., Bloom, F.E.: The catecholamine-containing neurons in the cat dorsolateral pontine tegmentum: Distribution of the cell bodies and some axonal projections. Brain Research 66, 1–21 (1974)Google Scholar
  8. Commissiong, J.W., Hellström, S.O., Neff, N.H.: A new projection from locus coeruleus to the spinal ventral columns: histochemical and biochemical evidence. Brain Research 148, 207–213 (1978)Google Scholar
  9. Corrodi, H., Fuxe, K., Hamberger, B., Ljungdahl, Å.: Studies on central and peripheral noradrenaline neurons using a new dopamine-β-hydroxylase inhibitor. Eur. J. Pharmacol. 12, 145–155 (1970)Google Scholar
  10. Crutcher, K.A., Humbertson Jr., A.O., Martin, G.F.: The origin of brainstem-spinal pathways in the North American opossum (Didelphis virginiana). Studies using the horseradish peroxidase method. J. Comp. Neurol. 179, 169–194 (1978)Google Scholar
  11. Dahlström, A., Fuxe, K.: Evidence for the existence of monoamine-containing neurons in the central nervous system. Acta physiol. scand. 62, 1–55 (1964)Google Scholar
  12. Eller, T., Chan-Palay, V.: Afferents to the cerebellar lateral nucleus. Evidence from retrograde transport of horseradish peroxidase after pressure injections through micropipettes. J. Comp. Neurol. 166, 285–302 (1976)Google Scholar
  13. Fuxe, K., Goldstein, M., Hökfelt, T., Hyub, J.T.: Immunohistochemical localization of dopamine-β-hydroxylase in the peripheral and central nervous system. Res. Commun. Chem. Pathol. Pharmacol. 1, 627–636 (1970)Google Scholar
  14. Graham, R.C., Karnovsky, M.J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966)Google Scholar
  15. Hökfelt, T., Fuxe, K.: Cerebellar monoamine nerve terminals, a new type of afferent fibers to the cortex cerebelli. Exp. Brain Res. 9, 63–72 (1969)Google Scholar
  16. Jacobs, B.L., Foote, S.L., Bloom, F.E.: Differential projections of neurons within the dorsal raphe nucleus of the rat: a horseradish peroxidase (HRP) study. Brain Research 147, 149–153 (1978)Google Scholar
  17. Jones, B.E., Moore, R.Y.: Catecholamine-containing neurons of the nucleus locus coeruleus in the cat. J. Comp. Neurol. 157, 43–52 (1977a)Google Scholar
  18. Jones, B.E., Moore, R.Y.: Ascending projections of the locus coeruleus in the rat. II. Autoradiographic study. Brain Research 127, 23–53 (1977b)Google Scholar
  19. Jones, B.E., Halaris, A.E., McIlhany, M., Moore, R.Y.: Ascending projections of the locus coeruleus in the rat. I. Axonal transport in central noradrenaline neurons. Brain Research 127, 1–21 (1977)Google Scholar
  20. Kobayashi R.M., Palkovits, M., Kopin, I.J., Jacobowitz, D.M.: Biochemical mapping of noradrenergic nerves arising from the rat locus coeruleus. Brain Research 77, 269–279 (1974)Google Scholar
  21. Korf, J., Aghajanian, G.K., Roth, R.H.: Stimulation and destruction of the locus coeruleus: Opposite effects on 3-methoxy-4-hydroxyphenylglycol sulfate levels in the rat cerebral cortex. Eur. J. Pharmacol. 21, 305–310 (1973)Google Scholar
  22. Kotchabhakdi, N., Hoddevik, G.H., Walberg, F.: Cerebellar afferent projections from the perihypoglossal nuclei: An experimental study with the method of retrograde axonal transport of horseradish peroxidase. Exp. Brain Res. 31, 13–29 (1978)Google Scholar
  23. Kuypers, H.G.J.M., Maisky, V.A.: Retrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat. Neuroscience Letters 1, 9–14 (1975)Google Scholar
  24. Larsell, O.: The comparative anatomy and histology of the cerebellum from monotremes through apes (J. Jansen, ed.), pp. 269. Minneapolis: University of Minnesota Press 1970Google Scholar
  25. Loizou, L.A.: Projections of the nucleus locus coeruleus in the albino rat. Brain Research 15, 563–566 (1969)Google Scholar
  26. Maeda, T., Pin, C.: Organisation et projections des systèmes catécholaminergiques du pont chez le chaton. C.v. Soc. Biol. (Paris) 165, 2137–2141 (1971)Google Scholar
  27. Maeda, T., Pin, C., Salvert, D., Ligier, M., Jouvet, M.: Les neurones contenant des catécholamines du tegmentum pontique et leurs voies de projections chez le chat. Brain Research 57, 119–152 (1973)Google Scholar
  28. Maisky, V.H., Kuypers, H.: Studies on the supraspinal system neurons in the cat brain by the technique of retrograde axonal transport of horseradish peroxidase. Nevrophysiologia (Kiev), 10, 115–124 (1978a)Google Scholar
  29. Maisky, V.H., Kuypers, H.: Studies on pathways in the cat spinal cord by the technique of retrograde axonal transport of horseradish peroxidase. Nevrophysiologia (Kiev), 10, 125–132 (1978b)Google Scholar
  30. McBride R.L., Sutin, J.: Projections of the locus coeruleus and adjacent pontine tegmentum in the cat. J. Comp. Neurol. 165, 265–284 (1976)Google Scholar
  31. Nygren, L.-G., Olson, L.: A new major projection from locus coeruleus: The main source of noradrenergic nerve terminals in the ventral and dorsal columns of the spinal cord. Brain Research 132, 85–93 (1977)Google Scholar
  32. Olson, L., Fuxe, K.: On the projections from the locus coeruleus noradrenaline neurons: The cerebellar innervation. Brain Research 28, 165–171 (1971)Google Scholar
  33. Pickel, V.M., Krebs, H., Bloom, F.E.: Proliferation of norepinephrine-containing axons in rat cerebellar cortex after peduncle lesions. Brain Research 59, 169–179 (1973)Google Scholar
  34. Pickel, V.M., Segal, M., Bloom, F.E.: A radioautographic study of the efferent pathways of the nucleus locus coeruleus. J. Comp. Neurol. 155, 15–42 (1974)Google Scholar
  35. Pickel, V.M., Joh, T.H., Reis, D.J.: A serotonergic innervation of noradrenergic neurons in nucleus locus coeruleus: Demonstration by immunocytochemical localization of the transmitter specific enzymes tyrosine and tryptophan hydroxylase. Brain Research, 131, 197–214 (1977)Google Scholar
  36. Pierce, E.T., Hoddevik, G.H., Walberg, F.: The cerebellar projection from the raphe nuclei in the cat as studied with the method of retrograde transport of horseradish peroxidase. Anat. Embryol. 152, 73–87 (1977)Google Scholar
  37. Poitras, D., Parent, A.: Atlas of the distribution of monoamine-containing nerve cell bodies in the brain stem of the cat. J. Comp. Neurol. 179, 699–718 (1978)Google Scholar
  38. Ross, R.A., Reis, D.J.: Effects of lesions of locus coeruleus on regional distribution of dopamine-β-hydroxylase activity in rat brain. Brain Research 73, 161–166 (1974)Google Scholar
  39. Russell, G.V.: The nucleus locus coeruleus (dorsolateralis tegmenti). Tex. Rep. Biol. Med. 13, 939–988 (1955)Google Scholar
  40. Sapawi, R.R., Divac, I.: Connections of the mediodorsal nucleus of the thalamus in the tree shrew. I. Afferent connections. Neuroscience Letters 7, 183–189 (1978)Google Scholar
  41. Shimizu, N., Ohnishi, S., Tohyama, M., Maeda, T.: Demonstration by degeneration silver method of the ascending projection from the locus ceruleus. Exp. Brain Res. 21, 181–192 (1974)Google Scholar
  42. Snider, R.S.: A cerebellar-ceruleus pathway. Brain Research. 88, 59–63 (1975)Google Scholar
  43. Swanson, L.W., Hartman, B.K.: The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine-β-hydroxylase as a marker. J. Comp. Neurol. 163, 467–506 (1975)Google Scholar
  44. Ungerstedt, U.: Stereotaxic mapping of the monoamine pathways in the rat brain. Acta physiol. scand. Suppl. 367, 1–48 (1971)Google Scholar
  45. Walberg, F., Brodal, A., Hoddevik, G.H.: A note on the method of retrograde transport of horseradish peroxidase as a tool in studies of afferent cerebellar connections, particularly those from the inferior olive; with comments on the orthograde transport in Purkinje cell axons. Exp. Brain Res. 24, 383–401 (1976)Google Scholar
  46. Wenzel, J., Wenzel, C.: De penitiori structura cerebri, p. 168. Tubing 18/2Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • R. Somana
    • 1
  • F. Walberg
    • 1
  1. 1.Anatomical InstituteUniversity of OsloOsloNorway
  2. 2.Laboratory of Neurobiology, Faculty of ScienceMahidol UniversityBangkokThailand

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