The yeast Yarrowia lipolytica has two, functional, signal recognition particle 7S RNA genes
Original Articles
Received:
Summary
Cells containing a deletion of either the SCR1 or SCR2 genes, which code for the 7SL RNA component of the signal recognition particle (SRP) homologue, were found to be viable. Two independent approaches demonstrated that cells containing deletions of both genes were inviale. Therefore, Yarrowia lipolytica contains two (and only two) functional 7SL RNA genes.
Key words
Yarrowia lipolytica 7SL RNA Essential genes YeastPreview
Unable to display preview. Download preview PDF.
References
- Barth G, Weber H (1985) Antonie van Leeuwenhoek 51:167–177Google Scholar
- Beckerich JM, Fournier P, Gaillardin C, Heslot H, Rochet M, Treton B (1984) In: Ball C (ed) Genetics and breeding of industrial microorganisms. CRC Press, Boca Raton, Florida, pp 115–157Google Scholar
- Brennwald P, Liao X, Holm K, Porter G, Wise JA (1988) Mol Cell Biol 8:1580–1590Google Scholar
- Campos N, Palau J, Torrent M, Ludevid D (1988) J Biol Chem 263:9646–9650Google Scholar
- Campos n, Palau J, Zwieb C (1989) Nucleic Acids Res 17:1573–1588Google Scholar
- Davidow LS, Apostolakos D, O'Donnell MM, Proctor AR, Ogrydziak DM, Wing RA, Stasko I, DeZeeuw JR (1985) Curr Genet 10:39–48Google Scholar
- Davidow LS, Kaczmarek FS, DeZeeuw JR, Conlon SW, Lauth MR, Pereira DA, Franke AE (1987) Curr Genet 11:377–388Google Scholar
- Gaillardin C, Charoy V, Heslot H (1973) Arch Mikrobiol 92:69–83Google Scholar
- Gundelfinger ED, Di Carlo M, Zopf D, Melli M (1984) EMBO J 3:2325–2332Google Scholar
- He F, Beckerich JM, Ribes V, Tollervey D, Gaillardin CM (1989) Curr Genet 16:347–350Google Scholar
- Liao X, Brennwald P, Wise JA (1989) Proc Natl Acad Sci USA 86:4137–4141Google Scholar
- Maniatis T, Fritch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
- Ogrydziak D, Bassel J, Contopulou R, Mortimer R (1978) Mol Gen Genet 163:229–239Google Scholar
- Poritz M, Siegel V, Hansen W, Walter P (1988) Proc Natl Acad Sci USA 85:4315–4319Google Scholar
- Reed KC, Mann DA (1985) Nucleic Acids Res 13:7207–7221Google Scholar
- Ribes V, Dehoux P, Tollervey D (1988) EMBO J 7:231–237Google Scholar
- Rothstein RJ (1983) Methods Enzymol 101:202–211Google Scholar
- Sherman F, Fink GR, Hicks JB (1986) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
- Siegel V, Walter P (1985) J Cell Biol 100:1913–1921Google Scholar
- Siegel V, Walter P (1988) Cell 52:39–49Google Scholar
- Ullu E, Murphy S, Melli M (1982) Cell 29:195–202Google Scholar
- Walter P, Blobel G (1980) Proc Natl Acad Sci USA 77:7112–7116Google Scholar
- Walter P, Blobel G (1981) J Cell Biol 91:557–561Google Scholar
- Walter P, Blobel G (1982) Nature 299:691–698Google Scholar
- Walter P, Lingappa V (1986) Annu Rev Cell Biol 2:499–516Google Scholar
- Xuan JW, Fournier P, Gaillardin C (1988) Curr Genet 14:15–21Google Scholar
Copyright information
© Springer-Verlag 1990