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Current Genetics

, Volume 17, Issue 1, pp 1–6 | Cite as

Heterologous expression of human 5-aminolevulinate dehydratase in Saccharomyces cerevisiae

  • Wren E. Schauer
  • James R. Mattoon
Original Articles

Summary

A cDNA coding for human 5-aminolevulinate dehydratase was placed in a yeast expression vector under the control of the GAL10 promoter. The resulting multicopy plasmid was then used to transform a yeast mutant which contains a defective hem2 gene coding for 5-aminolevulinate dehydratase. Expression of the human cDNA was shown in four ways: (1) restoration of normal growth on glycerol/galactose as primary carbon source, (2) decrease in intracellular 5-aminolevulinic acid concentration, (3) restoration of cytochrome biosynthesis and (4) direct, in situ assay of 5-aminolevulinic acid dehydratase. Curing transformed cells of plasmid restored the hem2 mutant phenotype. This heterologous system could be used to produce large quantities of human 5-aminolevulinic acid dehydratase for physical and biochemical studies.

Key words

Human ALA dehydratase Yeast expression 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Wren E. Schauer
    • 1
  • James R. Mattoon
    • 1
  1. 1.Biotechnology CenterUniversity of ColoradoColorado SpringsUSA

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