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Cell and Tissue Research

, Volume 273, Issue 2, pp 227–237 | Cite as

Intralobular heterogeneity of perisinusoidal stellate cells in porcine liver

  • Kenjiro Wake
  • Tetsuji Sato
Article

Abstract

The aim of the present investigation was to elucidate the intralobular heterogeneity of the perisinusoidal stellate cells (fat-storing cells, lipocytes) in the porcine liver. Their three-dimensional structure, desmin immunoreactivity and vitamin-A storage were studied by use of the Golgi silver, immunocytochemical and gold chloride methods. In order to locate the stellate cells, the hepatic lobules were divided into 10 zones. The stellate cells were readily identified in Golgi preparations by their striking dendritic appearance with branching processes encompassing the sinusoids. The stellate cells in the centrolobular zones were conspicuously dendritic with longer processes in conspicuously dendritic with longer processes in comparison to those emitted by periportal elements. Such arborizations were studded with numerous thorn-like microprojections. Desmin immunoreaction in the periportal zones was stronger than that in the centrolobular zones. Vitamin-A storage in the stellate cells was well developed in zones 2–4, but reduced gradually toward the central region. The perisinusoidal etellate cells display marked heterogeneity in morphology and function based on their zonal location in the hepatic lobule.

Key words

Perisinusoidal stellate cells (fat-storing cells) Intralobular heterogeneity Desmin Vitamin A Golgi method Liver Pig 

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Copyright information

© Springer-Veerlag 1993

Authors and Affiliations

  • Kenjiro Wake
    • 1
  • Tetsuji Sato
    • 1
  1. 1.Department of Anatomy, Faculty of MedicineTokyo Medical and Dental UniversityTokyoJapan

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