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Current Genetics

, Volume 23, Issue 5–6, pp 450–454 | Cite as

Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyotyping and hybridization with cloned genes

  • Elena Naumova
  • Gennadi Naumov
  • Philippe Fournier
  • Huu-Vang Nguyen
  • Claude Gaillardin
Original Articles

Abstract

Significant differences in electrophoretic karyotyping patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains showed unique karyotypes. Chromosomal DNAs of different strains were hybridized with cloned genes of Y. lipolytica (URA3, LEU2, ARS18 and ARS68), which revealed four different bands in most strains. We conclude that the haploid chromosome number of Y. lipolytica is at least four, and possibly five or six. Electrophoretic karyotyping and hybridization with cloned genes of Y. lipolytica provided evidence of a large divergence between Y. lipolytica and related species of Saccharomycopsis, Endomycopsella and Endomyces.

Key words

Karyotype Yarrowia lipolytica Chromosome polymorphism Hybridization 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Elena Naumova
    • 1
  • Gennadi Naumov
    • 1
  • Philippe Fournier
    • 2
  • Huu-Vang Nguyen
    • 2
  • Claude Gaillardin
    • 2
  1. 1.State Institute for Genetics and Selection of Industrial MicroorganismsMoscowRussia
  2. 2.Collection de Levures d'Intérêt Biotechnologique INRA-INA-CNRS, Centre de Biotechnologies Agro-IndustriellesInstitut National AgronomiqueThiverval-GrignonFrance

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