Current Genetics

, Volume 23, Issue 5–6, pp 392–396

Phenotypic identification of amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae

  • Michael J. Dorsey
  • Paula Hoeh
  • Charlotte E. Paquin
Original Articles


Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.

Key words

Saccharomyces cerevisiae Gene amplification ADH4 CUP1 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Michael J. Dorsey
    • 1
  • Paula Hoeh
    • 1
  • Charlotte E. Paquin
    • 1
  1. 1.Department of Biological Sciences, ML 006University of CincinnatiCincinnatiUSA

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