Current Genetics

, Volume 23, Issue 5–6, pp 392–396

Phenotypic identification of amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae

  • Michael J. Dorsey
  • Paula Hoeh
  • Charlotte E. Paquin
Original Articles

Abstract

Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.

Key words

Saccharomyces cerevisiae Gene amplification ADH4 CUP1 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aladjem MI, Koltin Y, Lavi S (1988) Mol Gen Genet 211:88–94Google Scholar
  2. Anderson RP, Roth J (1977) Annu Rev Micro 31:473–505Google Scholar
  3. Becker DM, Guarente L (1991) High-efficiency transformation of yeast by electroporation. In: Guthrie C, Fink G (eds) Guide to yeast genetics and molecular biology. Academic Press, New York, pp 182–187Google Scholar
  4. Berns EMJJ, Klijn JGM, van Putten WLJ, van Staveren IL, Portengen H, Foekens JA (1992) Cancer Res 52:1107–1113Google Scholar
  5. Bishop JM (1991) Cell 64:235–248Google Scholar
  6. Boeke JD, Trueheart J, Natsoulis G, Fink GR (1987) Methods Enzymol 154:164–175Google Scholar
  7. Botstein D, Carl Falco S, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW (1979) Gene 8:17–24Google Scholar
  8. Bridges CB (1936) Science 83:210–211Google Scholar
  9. Carle GF, Olson MV (1985) Proc Natl Acad Sci USA 82:3756–3760Google Scholar
  10. Chu G, Vollrath D, Davis RW (1986) Science 234:1582–1585Google Scholar
  11. Ciriacy M (1976) Mol Gen Genet 145:327–333Google Scholar
  12. Ciriacy M (1979) Mol Gen Genet 176:427–431Google Scholar
  13. Denis C (1984) Genetics 108:833–844Google Scholar
  14. Denis CL, Young ET (1983) Mol Cell Biol 3:360–370Google Scholar
  15. Dorsey M, Peterson C, Bray K, Paquin CE (1992) Genetics 132: 943–950Google Scholar
  16. Fogel S, Welch JW (1982) Proc Natl Acad Sci USA 79:5342–5346Google Scholar
  17. Hansche PE, Beres V, Lange P (1978) Genetics 88:673–687Google Scholar
  18. Hightower RC, Santi DV (1989) Electrophoresis 10:283–290Google Scholar
  19. Karin M, Najarian R, Haslinger A, Valenuela P, Welch J, Fogel S (1984) Proc Natl Acad Sci USA 81:337–341Google Scholar
  20. Kimura M, Ohta T (1974) Proc Natl Acad Sci USA 71:2848–2852Google Scholar
  21. Klessig DJ, Berry JO (1983) Plant Mol Biol Rep 1:12–18Google Scholar
  22. Link AJ, Olson MV (1991) Genetics 127:681–698Google Scholar
  23. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  24. Paquin CE, Williamson VM (1986) Mol Cell Biol 6:70–79Google Scholar
  25. Paquin CE, Dorsey M, Crable S, Sprinkel K, Sondej M, Williamson VM (1992) Genetics 130:263–271Google Scholar
  26. Slamon DJ, Godolphin W, Jones LA, Holt JA, Wong SG, Keith DE, Levin WJ, Stuart SG, Udove J, Ullrich A, Press MF (1989) Science 244:707–712Google Scholar
  27. Schimke RT (1984) Cell 37:705–713Google Scholar
  28. Sherman F (1991) Getting started with yeast. In: Guthrie C, Fink G (eds) Guide to yeast genetics and molecular biology. Academic Press, New York, pp 3–21Google Scholar
  29. Spector LM, Fogel S (1992) Curr Genet 21:309–318Google Scholar
  30. Stark GR (1983) Cancer Surveys 5:1–23Google Scholar
  31. Stark GR, Debatisse M, Giulotto E, Wahl GM (1989) Cell 57:901–908Google Scholar
  32. Tlsty TD (1990) Proc Natl Acad Sci USA 87:3132–3136Google Scholar
  33. Tlsty TD, Margolin H, Lum K (1989) Proc Natl Acad Sci USA 86:9441–9445Google Scholar
  34. Tlsty TD, White A, Sanchez J (1992) Science 255:1425–1427Google Scholar
  35. Walton J, Paquin C, Kaneko K, Williamson V (1986) Cell 46:857–863Google Scholar
  36. Welch J, Maloney DH, Fogel S (1990) Mol Gen Genet 222:304–310Google Scholar
  37. Whittaker SG, Rockmill BM, Blechl AE, Maloney DH, Resnick MA, Fogel S (1988) Mol Gen Genet 215:10–18Google Scholar
  38. Williamson VM, Young ET, Ciriacy M (1981) Cell 23:605–614Google Scholar
  39. Windle B, Draper BW, Yin Y, O'Gorman S, Wahl GM (1991) Genes Dev 5:160–174Google Scholar
  40. Wright JA, Smith HS, Watt FM, Hancock MC, Hudson DL, Stark GR (1990) Proc Natl Acad Sci USA 87:1791–1795Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Michael J. Dorsey
    • 1
  • Paula Hoeh
    • 1
  • Charlotte E. Paquin
    • 1
  1. 1.Department of Biological Sciences, ML 006University of CincinnatiCincinnatiUSA

Personalised recommendations