Current Genetics

, Volume 18, Issue 2, pp 169–170 | Cite as

An effective transformation method for Hansenula polymorpha

  • E. Berardi
  • D. Y. Thomas
Short Communications
Received:

Summary

We have developed a rapid, simple transformation procedure for intact cells of Hansenula polymorpha. It is a modification of the LiAc method and can yield 104–105 transformants/μg DNA. The use of stationaryphase cells, a high cell density per plate and a hear pulse at 50°C for 10 min are among major modifications of the original method. We have also found that, within the XhoI-SalI chromosomal fragment of Saccharomyces cerevisae containing the LEU2 gene, a sequence exists which supports autonomous replication of plasmid moleculles in H. polymorpha.

Key words

Transformation Hansenula polymorpha Thermotolerant yeasts Methylotrophic yeasts LEU2 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • E. Berardi
    • 1
  • D. Y. Thomas
    • 1
  1. 1.Genetic Engineering SectionBiotechnology Research InstituteMontréalCanada

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