Current Genetics

, Volume 18, Issue 2, pp 141–153 | Cite as

Patterns of organellar and nuclear inheritance among progeny of two geographically isolated strains of Volvox carteri

  • C. R. Adams
  • K. A. Stamer
  • J. K. Miller
  • J. G. McNally
  • M. M. Kirk
  • D. L. Kirk
Original Articles
Received:

Summary

Strains of Volvox carteri forma nagariensis derived from Japanese and Indian isolates (“J” and “I” strains, respectively) exhibited length differences (RFLPs) for approximately 90% of the restriction fragments detected by hybridization with a variety of unique-sequence, small-gene-family and repetitive-element probes, including heterologous probes of chloroplast and mitochondrial origin. Extensive post-zygotic mortality was observed among the zygotes produced by crossing J and I strains, suggesting some form of genetic incompatability between them. Most of the viable progeny exhibited recombinant patterns of nuclear inheritance and maternal inheritance of mitochondrial and chloroplast markers. However, many progeny exhibited exclusively uniparental (usually maternal, but in one case paternal) inheritance of both nuclear and organellar markers. Some of these non-recombinant individuals may be derived from “parthenospores” (dormant asexual cells resembling zygospores). Others may be a result of “pseudogamy,” in which one of the parental pronuclei is excluded from the zygote, followed by selective exclusion of both the mitochondrial and the chloroplast genomes derived from that same parent. When segregation patterns for 44 nuclear markers were analyzed in 90 recombinant progeny, statistically significant, locus-specific deviations from expected Mendelian transmission ratios were observed for a sizeable fraction of all markers in both reciprocal crosses: some markers were preferentially transmitted by the J strain, while others were preferentially transmitted by the I strain. It is speculated that these transmission distortions may be related to the regions of inter-isolate genetic incompatability, and may complicate the use of JxI crosses to establish a RFLP-based linkage map for the species.

Key words

Non-Mendelian inheritance Paternal inheritance Restriction fragment length polymorphism Volvox 
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References

  1. Bell G (1982) The masterpiece of nature; the evolution and genetics of sexuality. University of California Press, BerkeleyGoogle Scholar
  2. Bernatsky R, Tanksley SD (1986) Genetics 112:887–898Google Scholar
  3. Boynton JE, Harris EH, Burkhardt BD, Lamerson PM, Gillham NW (1987) Proc Natl Acad Sci USA 84:2391–2395Google Scholar
  4. Darden WH, Sayers ER (1969) Microbios 2:171–176Google Scholar
  5. Donis-Keller H, Green P, Helms C, Cartinhour S, Weiffenbach B, Stephans K, Keith TP, Bowden DW, Smith DR, Lander ES, Botstein D, Akots G, Rediker KS, Gravius T, Brown VA, Rising MB, Parkers C, Powers JA, Watt DE, Kaufmann ER, Bricker A, Phipps R, Muller-Kahle H, Fulton TR, Ng S, Schumm JW, Braman JC, Knowlton RG, Barker DF, Crooks SM, Lincoln SE, Daly MJ, Abrahamson J (1987) Cell 51:319–337Google Scholar
  6. Dover G, Brown S, Coen E, Dallas J, Strachan T, Trick M (1982) In: Dover GA, Flavell RB (eds) Genome evolution. Academic Press, New York, pp 343–372Google Scholar
  7. Drayna D, White R (1985) Science 230:753–758Google Scholar
  8. Ertl H, Mengele R, Wenzl S, Engel J, Sumper M (1990) J Cell Biol (in press)Google Scholar
  9. Gross CH, Ranum LPW, Lefebvre PA (1988) Curr Genet 13:503–508Google Scholar
  10. Harper JF (1985) Ph.D. thesis, Washington University, St Louis MoGoogle Scholar
  11. Harper JF, Huson KS, Kirk DL (1987) Genes Develop 1:573–584Google Scholar
  12. Harper JF, Mages W (1988) Mol Gen Genet 213:315–324Google Scholar
  13. Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J (1986) Theor Appl Genet 72:761–769Google Scholar
  14. Huskey RJ, Griffin BE (1979) Develop Biol 72:226–235Google Scholar
  15. Huskey RJ, Griffin BE, Cecil PO, Callahan AM (1979a) Genetics 91:229–244Google Scholar
  16. Huskey RJ, Semenkovich CF, Griffin BE, Cecil PO, Callahan AM, Chace KV, Kirk DL (1979b) Mol Gen Genet 169:157–161Google Scholar
  17. Jeffreys AJ, Brookfield JFY, Semeonoff R (1985) Nature 317:818–819Google Scholar
  18. Kirk DL (1988) Trends Genet 4:32–36Google Scholar
  19. Kirk DL (1990) In: Wiessner W, Robinson D (eds) Recent advances in experimental phycology, vol 5. Springer, Berlin Heidelberg New York (in press)Google Scholar
  20. Kirk DL, Baran GJ, Harper JF, Huskey RJ, Huson KS, Zagris N (1987) Cell 48:11–24Google Scholar
  21. Kirk DL, Harper JF (1986) Int Rev Cytol 99:217–293Google Scholar
  22. Kirk DL, Kirk MM (1983) Develop Biol 96:493–506Google Scholar
  23. Lee RW, Langille B, Lemieux C, Boer PH (1990) Curr Genet 17:73–76Google Scholar
  24. Mages H-W, Tschochner H, Sumper M (1988a) FEBS Lett 234:407–410Google Scholar
  25. Mages W, Salbaum JM, Harper JF, Schmitt R (1988b) Mol Gen Genet 213:449–458Google Scholar
  26. Müller K, Schmitt R (1988) Nucleic Acids Res 16:4121–4136Google Scholar
  27. Ranum LPW, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Genetics 120:109–122Google Scholar
  28. Rommens JM, Iannuzzi MC, Kerem B-S, Drumm ML, Melmer G, Dean M, Rozmahel R, Cole JL, Kennedy D, Hikada N, Zsiga M, Buchwald M, Riordan JR, Tsui L-C, Collins FS (1989) Science 245:1059–1065Google Scholar
  29. Sessoms AH, Huskey RJ (1973) Proc Natl Acad Sci USA 70:1335–1338Google Scholar
  30. Starr RC (1968) Proc Natl Acad Sci USA 59:1082–1088Google Scholar
  31. Starr RC (1969) Arch Protistenkd 111:204–222Google Scholar
  32. Starr RC (1970) Develop Biol (Suppl) 4:59–100Google Scholar
  33. Starr RC, Jaenicke L (1989) In: Coleman AW, Goff LJ, Stein-Taylor JR (eds) Algae as experimental systems. AR Liss, New York, pp 135–147Google Scholar
  34. St. George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Meyers RH, Feldman RG, Pollen D, Drachman D, Growdon J, Bruni A, Foncin J-F, Salmon D, Frommelt P, Amaducci L, Sorbi S, Piacentini S, Stewart GD, Hobbs WJ, Conneally PM, Gusella JF (1987) Science 235:885–890Google Scholar
  35. Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) Biotechnology 7:257–264Google Scholar
  36. Tschochner H, Lottspeich F, Sumper M (1987) EMBO J 6:2203–2207Google Scholar
  37. Vassart G, Georges M, Monsieur R, Brocas H, Lequarre AS, Christophe D (1987) Science 235:683–684Google Scholar
  38. Youngblom J, Schloss JA, Silflow CD (1984) Mol Cell Biol 4:2686–2696Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • C. R. Adams
    • 1
  • K. A. Stamer
    • 1
  • J. K. Miller
    • 1
  • J. G. McNally
    • 1
  • M. M. Kirk
    • 1
  • D. L. Kirk
    • 1
  1. 1.Department of BiologyWashington UniversitySt. LouisUSA

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