Current Genetics

, Volume 27, Issue 5, pp 440–446 | Cite as

Molecular cloning of the meiosis-induced rec10 gene of Schizosaccharomyces pombe

  • Yukang Lin
  • Gerald R. Smith
Original Paper


The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The nucleotide sequence of 3.5 kb of this clone revealed an open reading frame (ORF) encoding a 791 amino-acid polypeptide for the rec10 gene product. During meiosis, thermally induced in a temperature-sensitive pat1-114 mutant, the transcript of rec10 was induced to a maximal level at 2–3 h but was present at much lower levels before and after this time. The transient induction of the rec10 transcript and the rec10 mutant phenotype suggest that the rec10 gene product is involved primarily in the early steps of meiotic recombination localized to chromosome III in S. pombe.

Key words

Schizosaccharomyces pombe rec10 Transcription Melosis Recombination 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool J Mol Biol 215:403–410Google Scholar
  2. Andrews BJ, Herskowitz I (1990) Regulation of cell cycle-dependent gene expression in yeast. J Biol Chem 265:14057–14060Google Scholar
  3. Bähler J, Schuchert P, Grimm C, Kohli J (1991) Synchronized meiosis and recombination in fission yeast: observations with part1-114 diploid cells. Curr Genet 19:445–451Google Scholar
  4. Broach JR, Hicks JB, Strathern JN (1979) Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8:121–133Google Scholar
  5. Calvin NM, Hanawalt PC (1988) High-efficiency transformation of bacterial cells by electroporation. J Bacteriol 170:2796–2801Google Scholar
  6. Cottarel G, Beach D, Deuschle U (1993) Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr Genet 23:547–548Google Scholar
  7. DeVeaux LC, Smith GR (1994) Region-specific activators of meiotic recombination in Schizosaccharomyces pombe. Genes Dev 8: 203–210Google Scholar
  8. DeVeaux LC, Hoagland NA, Smith GR (1992) Seventeen complementation groups of mutations decreasing meiotic recombination in Schizosaccharomyces pombe. Genetics J 30:251–262Google Scholar
  9. Elliott S, Chang C-W, Schweingruber ME, Schaller J, Rickli EE, Carbon J (1986) Isolation and characterization of the structural gene for secreted acid phosphatase from Schizosaccharomyces pombe. J Biol Chem 261:2936–2941Google Scholar
  10. Fan J, Chikashige Y, Smith CL, Niwa O, Yanagida M, Cantor CR (1988) Construction of a NotI restriction map of the fission yeast Schizosaccharomyces pombe genome. Nucleic Acids Res 17: 2801–2818Google Scholar
  11. Gordon CB, Campbell JL (1991) A cell cycle-responsive transcriptional control element and a negative control element in the gene encoding DNA polymerase α in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 88:6058–6062Google Scholar
  12. Grant SGN, Jessee J, Bloom FR, Hanahan D (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci USA 87: 4645–4649Google Scholar
  13. Grimm C, Kohli J, Murray J, Maundrell K (1988) Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet 215:81–86Google Scholar
  14. Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359Google Scholar
  15. Iino Y, Yamamoto M (1985) Mutants of Schizosaccharomyces pombe which sporulate in the haploid state. Mol Gen Genet 198:416–421Google Scholar
  16. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153: 163–168Google Scholar
  17. Kohli J (1987) Genetic nomenclature and gene list of the fission yeast Schizosaccharomyces pombe. Curr Genet 11:575–589Google Scholar
  18. Lin Y, Smith GR (1994) Transient, meiosis-induced expression of the rec6 and rec12 genes of Schizosaccharomyces pombe. Genetics 136:769–779Google Scholar
  19. Lin Y, Larson KL, Dorer R, Smith GR (1992) Meiotically induced rec7 and rec8 genes from Schizosaccharomyces pombe. Genetics 132:75–85Google Scholar
  20. Losson R, Lacroute F (1983) Plasmids carrying the yeast OMP decarboxylase structural and regulatory genes: transcription regulation in a foreign environment. Cell 32:371–377Google Scholar
  21. Lowndes NF, Johnson AL, Johnston LH (1991) Coordination of expression of DNA synthesis genes in budding yeast by a cell-cycle-regulated trans factor. Nature 350:247–250Google Scholar
  22. Lowndes NF, McInerny CJ, Johnson AL, Fantes PA, Johnston LH (1992) Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdc10 +. Nature 355:449–453Google Scholar
  23. Petes TD, Malone RE, Symington LS (1991) Recombination in yeast. In: Broach J, Jones E, Pringle J (eds) The molecular and cellular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 407–521Google Scholar
  24. Ponticelli AS, Smith GR (1989) Meiotic recombination-deficient mutants of Schizosaccharomyces pombe. Genetics 123:45–54Google Scholar
  25. Russell PR, Hall BD (1982) Structure of the Schizosaccharomyces pombe cytochrome c gene. Mol Cell Biol 2:106–116Google Scholar
  26. Smith GR (1988) Homologous recombination in procaryotes. Microbiol Rev 52:1–28Google Scholar
  27. Szankasi P, Smith GR (1992) A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe. J Biol Chem 267: 3014–3023Google Scholar
  28. Szankasi P, Heyer WD, Schuchert P, Kohli J (1988) DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe: wildtype and mutant alleles including the recombination hotspot allele ade6-M26. J Mol Biol 204:917–92Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Yukang Lin
    • 1
  • Gerald R. Smith
    • 1
  1. 1.Fred Hutchinson Cancer Research CenterSeattleUSA

Personalised recommendations