Current Genetics

, Volume 27, Issue 5, pp 435–439 | Cite as

Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae

  • S. Sakamoto
  • G. Tamura
  • K. Ito
  • T. Ishikawa
  • K. Iwano
  • N. Nishiya
Original Paper

Abstract

The cDNA encoding the endo-β-1,4-glucanase (carboxymethylcellulase; CMCase-I) from Aspergillus kawachii IFO 4308 was cloned. Nucleotide-sequence analysis of the cloned cDNA insert showed a 717-bp open reading frame that encoded a protein of 239 amino-acid residues. The predicted amino-acid sequence of the mature protein had considerable homology with the protein sequence of the FI-CMCase of Aspergillus aculeatus. The cDNA was introduced into Saccharomyces cerevisiae. The expressed enzyme had carboxylmethylcellulase acitivity, identified by clear zones on a CMC-agar plate after Congo Red staining.

Key words

Cellulase Aspergillus kawachii cDNA cloning Expression 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • S. Sakamoto
    • 1
  • G. Tamura
    • 1
  • K. Ito
    • 2
  • T. Ishikawa
    • 2
  • K. Iwano
    • 2
  • N. Nishiya
    • 2
  1. 1.Research Institute of Brewing Resources Co., Ltd.TokyoJapan
  2. 2.National Research Institute of BrewingTokyoJapan

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